The preliminary application of in situ hybridization in detecting proto-oncogenes expression in human leukemic cells |
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| Authors: | Zhao Lian Peng Miao Du Xinxu Chen Shurong Cai Jingren Li Xiusong Zhang Fenqin Wang Zhenyi Wang Dunrui Wang Xiaogang Chen Shishu |
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| Affiliation: | (1) Institute of Haematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai, China;(2) Department of Biochemistry, Shanghai Second Medical University, Shanghai, China |
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| Abstract: | An in situ hybridization technique with35S labelled proto-oncogene probes (c-myc & c-fes) was used to detect their expression in bone marrow cells of 22 cases of leukemia of various types and immature granulocytes and erythroblasts of 16 nomal myelograms as controls. Both c-myc and c-fes were detectable in leukemic cells as well as in immature granulocytes and erythroblasts of normal bone marrow, but the expression extent varied in different cases. The levels of c-myc expression in leukemic cells were higher than those in controls (P<0.001). There was no difference of c-fes expression in two groups of bone marrow cells (P>0.05). This technique provides us a new method in studying variations of proto-oncogene expression in leukemic cells. |
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