The preliminary application of in situ hybridization in detecting proto-oncogenes expression in human leukemic cells |
| |
Authors: | Zhao Lian Peng Miao Du Xinxu Chen Shurong Cai Jingren Li Xiusong Zhang Fenqin Wang Zhenyi Wang Dunrui Wang Xiaogang Chen Shishu |
| |
Affiliation: | (1) Institute of Haematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai, China;(2) Department of Biochemistry, Shanghai Second Medical University, Shanghai, China |
| |
Abstract: | An in situ hybridization technique with35S labelled proto-oncogene probes (c-myc & c-fes) was used to detect their expression in bone marrow cells of 22 cases of leukemia of various types and immature granulocytes and erythroblasts of 16 nomal myelograms as controls. Both c-myc and c-fes were detectable in leukemic cells as well as in immature granulocytes and erythroblasts of normal bone marrow, but the expression extent varied in different cases. The levels of c-myc expression in leukemic cells were higher than those in controls (P<0.001). There was no difference of c-fes expression in two groups of bone marrow cells (P>0.05). This technique provides us a new method in studying variations of proto-oncogene expression in leukemic cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|