不同离体时间和保存液对犬离体牙牙周膜细胞活力影响的实验研究

目的:探讨完全脱位牙不同离体时间和保存液对牙周膜细胞活力的影响。方法:麻醉拔除犬牙35个,首先将20个牙随机分为5组,分别为室温干燥放置0、30、60、120、240 min组,另15个牙室温干燥放置30 min后,随机分为3组,分别放入牛奶、HBSS液,100 g/L蜂胶液中浸泡2 h。各组处理完成后,采用全牙消化法获得牙周膜细胞,并通过4 g/L台盼蓝染色法检测各组牙周膜活细胞数和存活率。结果:室温干燥放置30、60、120、240 min后,牙周膜细胞存活率依次为33.6%、23.6%、18.5%、0.8%,而0 min的牙周膜细胞存活率可达95.5%。拔后30 min,经牛奶、HBSS液和100 g/L蜂胶液中保存2 h后,牙周膜细胞均有活力,其细胞存活率大小依次为100 g/L蜂胶液、HBSS液和牛奶,其中100 g/L蜂胶液与HBSS液相比无统计学差异(P>0.05),但与牛奶组相比,均有统计学差异(P<0.05)。结论:随着离体时间延长,完全脱位牙根面牙周膜细胞活力明显下降。100 g/L蜂胶液和HBSS液保存犬牙牙周膜细胞活力优于牛奶液。

Effects of different extra-oral storage time and storage medium on the viability of periodontal ligament cells from avulsed dog teeth

AIM:To evaluate the effects of different extra-oral storage time and storage medium on the viability of periodontal ligament cells from avulsed dog teeth.METHODS:Thirty-five dog teeth were extracted under local anesthesia and were randomly assigned to different treatment groups.Twenty teeth were stored at room temperature for 0,30,60,120,240 min,with 4 teeth in each time point group.The PDL cells were subsequently isolated by direct enzymatic digestion method.The viabilities of the cells were examined by 0.4% Trypan Blue Stains.The other 15 teeth were stored at room temperature for 30 min.Then they were divided into HBSS,milk and 10% Propolis group(with 5 teeth in each group) and stored in the respective medium for 2 hours.Cell viability were assessed in the same way.RESULTS: The number of viable PDL cells decreased with the increase of storage time.At 0,30,60,120 and 240 minutes,the survival rate of PDL cells was 95.5%,33.6%,23.6%,18.5% and 0.8% respectively.The viability of PDL cells from teeth stored in 10% Propolis showed higher viability than those in HBSS and milk.There was no significant difference between 10% Propolis and HBSS,however,significant difference was observed between milk and other groups.CONCLUSION:The number of viable PDL cells remarkably decreases with the increase of dry storage time.10% Propolis and HBSS are better in maintaining PDL cell viability than milk.