人类β-防御素在正常、深龋和炎症牙髓中的表达

目的:研究人类β-防御素(human beta-defensins,HBD)在正常、深龋和炎症牙髓中的表达及其意义。方法:采用免疫组化SP法分别对正常、深龋及炎症牙髓中β-防御素HBD-1、HBD-4的表达进行组织学定位,并通过Image pro-plus 5.1图像分析软件对HBD-1/HBD-4染色进行平均光密度值的测定。所得数据用SPSS 13.0统计软件进行相应的统计学分析。结果:在人牙髓中,β-防御素主要表达在成牙本质细胞的细胞核及细胞突起中。HBD-1在3组内表达无显著差异(P>0.05);HBD-4在深龋和牙髓炎组中的表达与正常组相比均显著增强(P<0.05),且炎症组亦强于深龋组,差异有统计学意义(P<0.05)。结论:HBD-1参与牙髓天然免疫系统;HBD-4参与龋病发展过程中牙髓组织的免疫防御反应。

Expression of human beta-defensins in dental pulp

AIM: To investigate the expression of human beta-defensins(HBD) in dental pulp so as to sexamine the role of HBD on dental pulp restoration after injury.METHODS: Immunohistochemical technique was applied to detect the expression and distribution of HBD(HBD-1/HBD-4) in human dental pulp at normal,deep caries and pulpitis conditions.IMAGE PRO-PLUS 5.1 software was used to quantify the optical density of the staining.Differences among the three groups were analyzed with SPSS 13.0 software.RESULTS: HBD1 and HBD4 was positively expressed in the odontoblast cells.There were no significant difference among HBD-1 expression in the three groups(P>0.05).Compared with the normal group,HBD-4 expression in deep caries and pulpitis groups was significantly up-regulated(P<0.05),furthermore significant difference was found between pulpitis group and deep caries group(P<0.05).CONCLUSION: HBD-1 may play an important role in the innate pulp immune system.HBD-4 may play a role in the adaptive immune system of dental pulp.