粉防己碱对新生大鼠心肌细胞低氧/复氧损伤中细胞凋亡的影响

目的 :研究粉防己碱对培养心肌细胞模拟缺血 /再灌过程中心肌细胞凋亡的影响 .方法 :采用SD大鼠乳鼠进行心肌细胞培养 ,建立模拟心肌缺血 /再灌注模型 ,实验分 3组 :①正常对照组 ;②模拟缺血 /再灌注组 :细胞缺氧 12 0min ,复氧 2 4 0min ;③ 30 μmol·L-1粉防己碱 +缺血 /再灌注组 .计算台盼蓝摄取率 ,测定乳酸脱氢酶 (LDH)活性 ,以流式细胞仪 (FCM)和透射电镜观察细胞凋亡 .结果 :在正常对照组 ,FCM未发现凋亡峰 ,电镜未发现凋亡细胞 ,而缺血 12 0min/再灌注 2 4 0min组FCM的凋亡率为 15 1% ,电镜可见到典型的凋亡细胞 ,台盼蓝摄取率和LDH活性显著增加 (P <0 0 1) .粉防己碱组则细胞大部分存活 ,凋亡率明显降低 (平均 3 2 % ) .结论 :细胞凋亡参与了心肌缺血 /再灌注损伤 ,粉防己碱可减少缺血 /再灌注损伤中的心肌细胞凋亡

Effects of tetrandrine on apoptosis of cultured neonatal rat cardiomyocytes during ischemia/reperfusion injury

AIM: To examine the effects of tetrandrine on the apoptosis of cultured neonatal rat cardiomyocytes during ischemia/reperfusion injury. METHODS: A cell culture model of neonatal rat cardiac myocytes was used. Three groups were adopted: control group, simulated ischemia/reperfusion group and the tetrandrine group. Apoptosis was determined by flow cytometry (FCM) and transmission electron microscope (TEM). RESULTS: Apoptosis was not found in the control group. After 120 min of simulated ischemia followed by 240 min of reperfusion, typical apoptotic cells were able to be seen by TEM and FCM showed an apoptosis rate of 15 1%. While in tetrandrine group, apoptosis rate was decreased to only 3 2%. CONCLUSION: Cardiomyocyte apoptosis is involved in the ischemia/reperfusion injury and tetrandrine can inhibit cardiomyocyte apoptosis during myocardial ischemia/reperfusion injury.