高渗盐己酮可可碱溶液对休克大鼠肺组织HSP70表达的影响

目的探讨己酮可可碱高渗盐溶液对失血性休克大鼠肺脏HSP70表达影响。方法 50只成年SD大鼠随机分为假手术组(Sham组)、休克组(Shock组)、乳酸林格液组(RL组)、高渗盐溶液组(HS组)、高渗盐己酮可可碱组(HS-PTX组)。复苏组SD大鼠在失血性休克1h后即分别使用RL、HS、HS-PTX行液体复苏疗法。休克复苏24h后,放血法处死大鼠,迅速打开胸腔取大鼠左肺,置入4%多聚甲醛固定,采用免疫组化来评估肺脏的HSP70表达水平,取其右肺匀浆,用Elisa方法检测肺组织内TNF-α和IL-6表达水平。结果 IL-6、TNF-α水平:HS-PTX组与HS组、RL组和Shock组比较,肺组织IL-6、TNF-α水平明显降低(P<0.05),较Sham组略显升高。肺组织HSP70表达水平测定:HS-PTX组较HS组、RL组和Shock组表达水平明显升高(P<0.05)。结论 HS-PTX可以减少IL-6、TNF-α的表达,对肺组织有保护作用,其作用机制可能与HSP70表达增加有关。

The Influence of HS-PTX on HSP70 Expression in Hemorrhagic Shock Induced Lung Injury in Rats

Objective To explore the influence of HS-PTX on HSP70 expression in hemorrhagic shock induced lung injuries in rats. Methods Fifty Sprague-Dawley rats were divided randomly into five groups： sham operational group （Sham）, shock group （Shock）, Ringer＇s lactated group （RL）, hypertonic saline group （HS） and HS-PTX group. Sprague-Dawley rats were bled to a mean arterial pressure of 35 mm Hg - 45 mm Hg for one hour. After shock, fluid resuscitation was operated with RL, HS and HS-PTX, respectively. Shed blood were infused after fluid resuscitation. Twenty-four hours after resuscitation, kill the rat the left lung was taken to test the level of TNF-α and IL-6 by ELISA. The right lung was taken to test the level of HSP70 by immunohistochemical. Results The level of TNF-α and IL-6 in the left lung by HS-PTX were lower than Shock group, HS group and RL group （P 〈0.05）, however, higher than Sham group. Positive expression of HSP70 in the right lung of HS-PTX group were lower than Shock group,HS group and RL group （P 〈0.05）. Conclusions HS-PTX protects the lung after hemorrhagic shock by increasing the level of HSP70.