智能多柱高效液相色谱系统及其在中药质量控制中的应用

高效液相色谱法在中药分析领域发挥了一定作用，但传统的单一色谱柱模式已无法满足现代中药研究中提供多成分乃至全成分信息的要求，智能多柱高效液相色谱系统是在多柱切换高效液相色谱与智能化技术的基础上提出的新概念，根据分析样品成分繁多、性质复杂的物点，利用切换技术的模块式分离性能，把样品分块地切换进不同性质的色谱柱，再用合适的流动相洗脱，全过程采用智能化控制，前期选择合适的色谱柱、流动相、操作条件，设计多柱色谱流路，中期控制多柱自动切换的精确性；后期对检测到的大量信息进行分析，去芜存真，得到可靠的定性定量结果。该系统的建立有望解决中药、生物体液、环境样品等复杂样品的快速、多成分分析。

Intellectual multi-column high performance liquid chromatographic system and its application in the quality control of traditional chinese medicine

High performance liquid chromatography(HPLC) has taken some effects in the analysis of traditional chinese medicine. But conventional single - column mode can't satisfy the increasing needs of providing multi or even whole components' imformation in the modem research of traditional chinese medicine. Intellectual multi - column high performance liquid chromatographic system(IMC - HPLG) is a new conception based on both column switching HPLC and intellectual technology. Due to the miscellaneousness of the analytical samples, sectional separation property of column switching technique is employed to cut the sample segmentally into different kinds of columns, and then, elute with suitable mobile phases. The whole procedure is controlled intellectually. In the earlier period, favorable solid/ mobile phase and chromatographic conditions are selected and multi - column flow diagram is designed. In the middle of the procedure, precision of the column - switching is controlled automatically. In the later stage, a great deal of imformation detected is analysed to eliminate the false and retain the true, to obtain the trustworthy qualitative and quantitative results. The system is expected to complete multi - component quick analysis of such complex samples as traditional chinese medicine, biological fluid and environmental specimen