VEGF反义核酸抑制人胰腺癌细胞的增殖

目的：探讨VEGF121反义核酸对体外培养胰腺癌细胞增殖的影响,以求证明VEGF反义核酸具有抑制肿瘤生长的作用。方法：将稳定转染有VEGF反义核酸pcDNA3-ASVEGF121的胰腺癌细胞系BxPC-3在体外条件下培养,采用ELISA法检测其培养上清液中的VEGF表达水平。检测肿瘤细胞生长曲线,通过克隆形成试验检测肿瘤细胞的体外增值能力。采用Annexin Ⅴ-FITC和PI双标记法以流式细胞仪检测肿瘤细胞凋亡情况。结果：VEGF反义核酸转染BxPC-3细胞后,肿瘤细胞培养液上清VEGF蛋白水平受到明显抑制。生长曲线显示肿瘤细胞生长明显受到抑制,并且肿瘤细胞克隆形成率降低。流式细胞仪检测显示VEGF反义核酸能促进细胞凋亡。结论：人反义VEGF121能显著抑制胰腺癌细胞的VEGF表达,并能抑制肿瘤细胞的生长,促进凋亡。

Human Antisense VEGF121 Expression Vector inhibits the proliferation of pancreatic cancer cell in vitro

Objective To explore the effect of antisense VEGF121 on cell proliferation in pancreatic cancer cell line in vitro, so as to elucidate the depression of tumor growth by antisense VEGF121. Methods Pancreatic Cancer cell BxPC- 3 was transfected with pcDNA3-ASVEGF121 and pcDNA3 as control. VEGF expression was tested by RT-PCR and ELISA. Colony forming test and growth curve MTT assay were carried out to assess the proliferation potency. Culture ceils were labeled by Annexin V-FITC and PI, then apoptosis was tested by flow cytometry. Results VEGF protein was decreased in the culture media from transfected cell. Cells transfected with antisense VEGF121 vector had a lower colony forming rate, while cell growth is significantly inhibited in the growth curve test, It was also put into evidence that antisense VEGF121 vector could promote apoptosis in the pancreatic cancer in vitro, Conclusions Antisense VEGF121 vector can inhibit the VEGF expression of pancreatic cancer cell line in vitro, and can inhibit cell growth as well as promote cell apoptosis,