MMP-7、PTEN在子宫内膜增生症及内膜样腺癌中的表达

目的研究基质金属蛋白酶-7(MMP-7)和抑癌基因(PTEN)在子宫内膜增生症至子宫内膜样腺癌组织中的异常表达及变化规律,探讨其作为子宫内膜早期癌变及浸润、转移的生物学标志的可能性。方法应用免疫组化S-P法检测15例正常增生期子宫内膜组织、40例子宫内膜增生症组织、38例子宫内膜样腺癌组织中MMP-7、PTEN的表达。结果在正常增生期子宫内膜,子宫内膜简单型、复杂型、不典型增生及子宫内膜样腺癌,MMP-7的阳性表达率呈递增趋势,PTEN呈递减趋势。等级相关分析结果显示:MMP-7、PTEN表达异常与癌组织学分级均显著相关(r分别为0.410和-0.569,P均<0.01)。除不典型增生外,正常增生期子宫内膜、子宫内膜简单型、复杂型增生与子宫内膜样腺癌组织中的MMP-7、PTEN表达差异有显著性。正常增生期子宫内膜、子宫内膜简单型增生与不典型增生比较,MMP-7、PTEN表达差异显著;在内膜样腺癌中,MMP-7与PTEN表达呈负相关(r=-0.392,P<0.01);MMP-7与肿瘤浸润深度呈正相关(r=0.389,P<0.05);PTEN蛋白表达与子宫内膜样腺癌的临床分期、组织学分级无关(P>0.05),与肌层浸润呈负相关(P<0.05)。结论MMP-7、PTEN蛋白在子宫内膜增生症至子宫内膜样腺癌组织中表达异常,两者可能成为子宫内膜组织早期癌变和预测生物学行为的重要分子标记物。

Expression of MMF-7 and PTEN in endometrial hyperplasia and endometrial adenocarcinoma

Objective To study the abnormal expression and varing regulations of MMP-7 and PTEN in endometrial hyperplasia to endometrial adenocarcinoma and investigate the possibilities of them as biomarkers of early carcinogenesis of endometria?transfer and infiltration of endometrial cancer.Methods To test the expresstions of MMP-7 and PTEN in 15 normal proliferative endometria?40 endometrial hyperplasia and 38 endometrial adenocarcinoma by immunohistochemical staining S-P method.Results The positive expression rates of MMP-7 increased gradually and those of PTEM decreased gradually in normal proliferative endometria?endometria of simple proliferation?endometria of complicated proliferation?endometria of nontypical proliferation and endometrial adenocarcinoma.The abnormal expressions of MMP-7 and PTEM were well both correlated with the pathological grading of adenocarcinoma.(r=0.410?-0.569,P<0.01).Except for endometria of nontypical proliferation,the expresstions of MMP-7 and PTEN in endometria of normal proliferation?endometria of simple proliferation?endometria of complicated proliferation were markedly different with and those of endometrial adenocarcinoma.The expressions of MMP-7 and PTEN in normal proliferative endometria and endometria of simple proliferation were different with those of nontypical proliferation.Negative correlation was found between the expresstions of MMP-7 and PTEN in endometrial adenocarcinom(r=-0.392,P<0.01).Positive correlation was between MMP-7 and the depth of infiltration of endometrial adenocarcinoma(r=0.389,P<0.05).No correlation was found between the expresstion of PTEN and operation grading?histological grading(P>0.05).Conclusions The expressions of MMP-7 and PTEN were abnormal in endometrial hyperplasia to endometrial adenocarcinoma.They may become important biomarkers of early carcinogenesis of endometria and predicting biological actions.