新辅助化疗对口腔鳞状细胞癌Survivin表达影响及意义

目的研究新辅助化疗对口腔鳞状细胞癌组织中Survivn蛋白表达的影响。方法选择18例活检病理确诊为口腔鳞癌的临床病例,予DFP方案行术前新辅助化疗2个疗程,分别取新辅助化疗前、后肿瘤组织,另取唇裂修补术中切除的多余的唇组织为口腔正常组织对照组10例,共3组,进行免疫组化染色检测Survivin表达。结果正常口腔组织、新辅助化疗前、后肿瘤组织Survivin阳性细胞表达率分别为(14.50±1.96)%、(57.78±3.39)%、(45.72±2.32)%,组间比较F=814.37,P<0.05。Survivin平均吸光度值为12.75±1.13、77.90±12.64、47.17±15.08,组间比较F=90.98,P<0.05。结论 Survivin在DFP方案新辅助化疗后表达显著降低。

Effect of neoadjuvant chemotherapy on Survivin expression in oral squamous cell carcinoma

Objective To investigate the effect of neoadjuvant chemotherapy on survivin expression in oral squamous cell carcinoma. Methods A total of 18 biopsy confirmed cases of OSCC were included. DFP program（cisplatin,5-fluorouracil,bleomycin） was adopted and two courses of neoadjuvant chemotherapy were performed before operation. Tumor tissue before and after neoadjuvant chemotherapy was got, and normal lip tissue needless in cheilorrhaphy cut off from 10 cases was regarded as control group of normal oral tissue. The expression of Survivin was analyzed in the three groups by immunohistochemistry. Results The expression rates of survivn positive cells in normal oral mucosa, OSCC before neoadjuvant chemotherapy and OSCC after neoadjuvant chemotherapy were （ 14.50 ± 1. 96 ） %, （ 57.78± 3.39 ） % and （45.72 ±2.32 ） % respectively （ P 〈 0.05 ）, with comparison among groups F = 814.37, P 〈 0.05. The average optical densities of Survivin in normal oral mucosa, OSCC before neoadjuvant chemotherapy and OSCC after neoadjuvant chemo- therapy were 12.75 ± 1.13,77.90 ± 12.64 and 47.17 ± 15.08 respectively, with comparison among groups F = 90.98, P 〈 0.05. Con- clusions The expression of Survivin decreases obviously after neoadjuvant chemotherapy（DFP program）.