Separation and analysis of saccharides--on mainly oligosaccharides

Characteristics of three kinds of columns used for the separation and analyses of saccharides by HPLC, and of a pulsed amperometric detector (PAD) as a high sensitive detector for the microanalysis of saccharides were described. The following several examples of studies on the separation and analyses of saccharides were illustrated: (1) separation of maltosaccharides on an amino column, (2) isolation of each maltooligomer of DP6-17 on an ODS column, (3) retention behavior of alpha-cyclodextrin (alpha CD) and branched alpha CDs on an ODS column, (4) separation of three positional isomers of dimaltosyl-beta CD on an ODS column and a graphitized carbon (GC) column, (5) separation of diastereoisomers of glucosyl-inositol on a GC column, (6) chromatographic behavior of nine glucobioses on a GC column, (7) estimation of the distributions of chain length of amylopectins with the pulsed amperometric detection, (8) microanalysis of beta CD and glucosyl-beta CD in biological matrices by HPLC with PAD. In addition, methods for the structural analyses of branched CDs by FAB-MS, 13C-NMR and enzymatic degradation were expressed.