| 斑马鱼胚胎发育过程中Mef2c的表达 |
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| 引用本文： | 刘学飞,王跃祥,蒋璆,钱林溪,高广文,董永新,钟涛,宋后燕.斑马鱼胚胎发育过程中Mef2c的表达[J].复旦学报(医学版),2006,33(1):89-91,F0002. |
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| 作者姓名： | 刘学飞 王跃祥 蒋璆 钱林溪 高广文 董永新 钟涛 宋后燕 |
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| 作者单位： | 1. 复旦大学上海医学院生物化学与分子生物学系-分子医学教育部重点实验室,上海,200032
2. 美国Vanderbilt大学医学院心血管研究室,TN 37027 |
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| 基金项目： | 国家自然科学基金;复旦大学校科研和教改项目 |
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| 摘 要： | 目的探讨Mef2c在斑马鱼胚胎骨骼肌和心肌中表达的时相变化.方法收集不同发育时期的AB野生型斑马鱼胚胎,采用整体原位杂交技术检测Mef2c在骨骼肌和心肌的转录情况.结果Mef2c在体节中的表达约在13 hpf,而在心脏中的表达出现在13 hpf以后,与体节中的表达时间基本平行.另外,Mef2c在体节和心脏中的表达一直维持到48 hpf.结论明确了Mef2c在斑马鱼胚胎骨骼肌和心肌发育的表达时相变化,为研究斑马鱼早期骨骼肌和心肌发育的调控机制提供了一定的理论基础.
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| 关 键 词： | 斑马鱼 反义RNA探针 整体原位杂交 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005年4月5日 |
Mef2c gene expression profile during zebrafish embryogenesis |
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| LIU Xue-fei,WANG Yue-xiang,JIANG Qiu,QIAN Lin-xi,GAO Guang-wen,DONG Yong-xin,ZHONG Tao,SONG Hou-yan.Mef2c gene expression profile during zebrafish embryogenesis[J].Fudan University Journal of Medical Sciences,2006,33(1):89-91,F0002. |
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| Authors: | LIU Xue-fei WANG Yue-xiang JIANG Qiu QIAN Lin-xi GAO Guang-wen DONG Yong-xin ZHONG Tao SONG Hou-yan |
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| Abstract: | Purpose To analyze the expression pattern of Mef2c during zebrafish embryogenesis. Methods We collected all the phases of zebrafish embryos to detect the expression pattern of Mef2c in the somite and the heart during zebrafish embryogenesis by whole mount in situ hybridization. Results We first detected Mef2c expression at 13 hours post-fertilization in the somite that is the latest expression in the somite of Mef2c family. Expression of Mef2c in heart was detected as early as in somite and continued for 48 hours post-fertilization. Conclusions We made clear the expression pattern of Mef2c during zebrafish embryogenesis in order to make sure that Mef2c plays important roles in the regulation of cardiac and skeletal muscle development. |
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| Keywords: | Mef2c |
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