非牙源性上皮与牙胚间充质相互作用诱导模式的筛选

目的：筛选非牙源性上皮向牙源性上皮转化过程中上皮-外胚间充质的最佳诱导模式。方法：体外培养并鉴定出生1d的SD仔鼠的牙胚外胚间充质细胞和富含干细胞的皮肤表皮细胞,并建立4种不同的表皮细胞与牙胚外胚间充质细胞间的诱导模式,通过组织学观察比较不同诱导模式所获得的重组体形成牙齿样结构的能力。结果：所采用的4组方案中,只有细胞-材料组和上皮外胚间充质混合细胞团块体内培养组形成了牙齿样结构,尤其是上皮外胚间充质混合细胞团块体内培养组所形成的牙齿样组织中同时含有牙本质和釉质样结构,而其他2组均无牙齿样结构的形成。结论：上皮外胚间充质混合细胞团块体内培养重组法最有利于牙齿组织的形成,是一种理想的研究非牙源性上皮与牙源性外胚间充质相互作用模式的诱导方法。

Selection of induction pattern for the interaction of nondental epithelium with dental papillae ectomesenchymal cells

AIM: To select a better induction pattern for the interactions of non-dental epithelium with dental papillae ecto-mesenchymal cells. METHODS: The skin epithelial cells (ECs) and the dental papillae ecto-mesenchymal cells (DPMCs) of 1day post-natal SD rats were cultured and identified. Four different induction patterns were designed between ECs and DPMCs. The recombinations were analyzed by histological analysis after 14 days culture. RESULTS: Tooth-like structures, which contained dentin and enamel, were formed after 14 days only in the ECs mixed with DPMCs after cultured in renal capsule of adults SD rat . Dentin-like structure formed when ECs mixed with DPMCs and seeded into PLGA scaffolds, but there was no enamel-like structure formation. There was no tooth-like structure formed in other groups. CONCLUSION: Preparation of ECs mixed with DPMCs used as cell pellets culture system was better than others, which was suitable for the interaction between non-dental epithelial cells to dental ecto-mesenchymal cells.