| 单细胞凝胶电泳技术在胚胎毒性实验中的应用 |
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| 引用本文： | 李树蕾,周莉,陈辉,黄可欣,聂毓秀,鄢励,刘颖丽. 单细胞凝胶电泳技术在胚胎毒性实验中的应用[J]. 吉林大学学报(医学版), 2002, 28(2): 124-126. DOI: 10.3969/j.issn.1671-587X.2002.02.005 |
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| 作者姓名： | 李树蕾 周莉 陈辉 黄可欣 聂毓秀 鄢励 刘颖丽 |
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| 作者单位： | 1. 吉林大学基础医学院组织胚胎学教研室,吉林,长春,130021;2. 吉林卫生学校组织胚胎学教研室;3. 吉林省妇幼保健院;4. 吉林省长春市医学高等专科学校 |
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| 基金项目： | 国家自然科学基金;29890282-3(2); |
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| 摘 要： | 目的:探讨和分析单细胞凝胶电泳技术(SCGE)的影响因素和实验中应该注意的环节.方法:通过对实验方法和操作技术的总结,对Singh提出的碱性SCGE进行适当的补充.结果:完善了SCGE的操作,获得了典型的彗星图像.结论:在实验中避免非特异性因素的影响,注意实验操作细节,能够增加SCGE的特异性和敏感性.
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| 关 键 词： | 单细胞凝胶电泳 胚胎毒性实验 应用 |
| 文章编号： | 1671-587X(2002)02-0124-03 |
| 修稿时间： | 2001-05-09 |
The application of single cell gel electrophoresis in experiment exmaing embryonal toxicity |
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| Abstract: | Objective:To investigated and analyzed the factors influencing on the results and the links attended. Methods:SCGE was modified properly during the test based on the basic SCGE advanced by Singh 1988 after summarizing experiment method and skill. Results:The operation of SCGE was perfected and the typical comet star was obtained. Conclusion:Specificity and sensitivity of SCGE would be enhanced if the effects of nonspecific factors were avoided and more attention was paid carefully during the assay. |
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| Keywords: | single cell gel electrophoresis embryonal toxicity application |
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