双项同测法测定血清丙氨酸转氨酶和天门冬氨酸转氨酶

目的 :研究一种可以同时测定血清丙氨酸转氨酶 (ALT)和天门冬氨酸转氨酶 (AST)的方法。方法 :用自配的双项同测试剂同时测定血清中的ALT和AST。结果 :双项同测试剂测定ALT和AST与成品试剂比较 ,相关性好(均r =0 .999) ;精密度高 ,批内变异均低于 5 % ;抗干扰能力强 ,溶血、黄疸、脂血不影响结果 ;检测速度较传统方法快一倍。结论 :双项同测法既能充分发挥仪器的功能又提高了测定速度 ,是一种值得推广的方法

Twin test in the determination of alanine aminotransferase and aspartate aminotransferase in the serum

Objective To explore a method which can simultaneously determine alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum. Methods Using the self prepared twin test reagent, ALT and AST in the serum were detected simultaneously. Results There was a good correlation between the twin test’s method and the traditional method in the detection of ALT and AST (All r=0.999). The precision of the method was good, and the coefficient variation (CV) was below 5%. Hemolysis, jaundice, and lipohemia serum were found to be interference free. The time of the detection was two times of the traditional method. Conclusion The twin test method can both exert the capability of the automatic analyzer and shorten the time of detection. It is a method worthy to be popularized.