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| High Glucose Promotes the CTGF Expression in Human Mesangial Cells via Serum and Glucocorticoid-induced Kinase 1 Pathway | |
| 作者姓名： | 王全胜 张阿丽 李仁康 刘建国 谢纪文 邓安国 冯玉锡 朱忠华 |
| 作者单位： | Department of Integrated Chinese Medicine and Western Medicine，Union Hospital，TongJi Medical College，Huazhong University of Science and Technology，Department of Radiotherapy and Chemotherapy，Zhongnan Hospital，Wuhan University，3Department of Nephrology，Union Hospital，TongJi Medical College，Huazhong University of Science and Technology |
| 摘 要： | The role of serum and glucocorticoid-induced kinase 1 （SGK1） pathway in the connective tissue growth factor （CTGF） expression was investigated in cultured human mesangial cells （HMCs） under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with PIRES2-EGFP- S422D hSGK1 （SD） could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with PIRES2-EGFP （FP） under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with PIRES2-EGFP- K127N hSGK1 （KN） could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs. |
| 关 键 词： | 高糖诱导 人肾小球系膜细胞 CTGF 蛋白激酶 激素诱导 糖皮质 结缔组织生长因子 通路 |
| 收稿时间： | 8 January 2009 |
High glucose promotes the CTGF expression in human mesangial cells via serum and glucocorticoid-induced kinase 1 pathway |
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| Quansheng?Wang,Ali?Zhang,Renkang?Li,Jianguo?Liu,Jiwen?Xie,Anguo?Deng,Yuxi?Feng,Zhonghua?Zhu.High Glucose Promotes the CTGF Expression in Human Mesangial Cells via Serum and Glucocorticoid-induced Kinase 1 Pathway[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(5):508-512. | |
| Authors: | Quansheng Wang Ali Zhang Renkang Li Jianguo Liu Jiwen Xie Anguo Deng Yuxi Feng Zhonghua Zhu |
| Institution: | 1. Department of Integrated Chinese Medicine and Western Medicine,Wuhan 430022, China 2. Department of Radiotherapy and Chemotherapy, Zhongnan Hospital Wuhan University, Wuhan 430071, China 3. Department of Nephrology, Union Hospital TongJi Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| Abstract: | The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with PIRES2-EGFP- S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with PIRES2-EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with PIRES2-EGFP- K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs. |
| Keywords: | high glucose serum and glucocorticoid-induced protein kinase 1 human mesangial cells connective tissue growth factor diabetic nephropathy |
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