Serological Identification of la Antigens: Report of a British Region la Workshop |
| |
Authors: | Julia Bodmer Penelope Pickbourne Walter Bodmer Richard Batchelor Patrick Dewar Heather Dick Colin Entwistle Hilliard Festenstein Keith Gelsthorpe Valerie Joysey Pauline Mackintosh Sylvia Lawler Peter Morris GD Pegrum Rodney Harris Malcolm Taylor |
| |
Institution: | Genetics Laboratory, Oxford;Queen Victoria Hospital, East Grinstead, Sussex;Blood Transfusion Centre, General Hospital, Newcastle upon Tyne;Bacteriology Department, Glasgow Royal Infirmary, Glasgow;NTTRL Blood Transfusion Centre, Southmead, Bristol;London Hospital, London, El 2AD;Regional Transfusion Centre, Sheffield;Tissue Typing Laboratory, Addenbrookes Hospital (Old Site), Cambridge;Regional Transfusion Service, Birmingham;Royal Marsden Hospital, London, SW3 6JJ;II Radcliffe Infirmary, Oxford;Charing Cross Hospital, London, W6 8RF;St. Mary's Hospital, Manchester, 13. |
| |
Abstract: | In preparation for the 7th International Histocompatibility Workshop 13 laboratories in the British Region participated in a local workshop. One hundred and twenty-three sera which had been previously shown to have activity on either normal B cells, CLL cells or B cell lymphoid lines in the absence of HLA-A, B or C activity were exchanged between the laboratories. These sera were tested on a total of 212 B cells, 101 CLL cells, 76 T cells and 76 lymphoid cell lines. The data was collected and analyzed in Oxford. The analysis showed that six groups of sera could be distinguished. When these groups were compared with the D locus typing of some of the lymphoid lines which were derived from individuals used as MLC typing cells, they were seen to have significant associations with D locus antigens. The serological groups defined were therefore given numbers corresponding to the D locus numbers they associate with, i.e. UK1 is associated with DW1 and so on for UK2, 3, 4, 5 and 7. Comparison of typing techniques showed that long incubation both with antiserum and then with complement, 1 hour + 2 hours gave the best and most reproducible reactions on normal B cells. Residual anti-HLA-A, B or C activity in some of the sera even after platelet absorption showed the importance of adequate checking on T cells after absorption. |
| |
Keywords: | |
|
|