辽宁产朝鲜淫羊藿不同药用部位有效成分含量的测定

目的测定辽宁不同产区朝鲜淫羊藿不同药用部位中淫羊藿苷及总黄酮的含量。方法以淫羊藿苷为指标，采用高效液相色谱法测定淫羊藿苷的含量，紫外分光光度法测定总黄酮的含量。结果叶中淫羊藿苷的含量在0．197％～0．347％范围内，茎中淫羊藿苷的含量在0．043％～0．050％范围内；叶中总黄酮的含量在6．68％～8．850范围内，茎中总黄酮的含量在1．3％～2．7％范围内。淫羊藿苷在0．3～1．8μg（r=0．9999）和0．5～3μg／mL（r=0．9999）范围内线性关系良好。淫羊藿苷的平均回收率为99．43％（n=6，RSD=0．29％），总黄酮为100．38％（n=9，RSD=1．60％）。结论辽宁不同地区的朝鲜淫羊藿有效成分含量不同，不同药用部位的总黄酮和淫羊藿苷含量差异较大，叶中的总黄酮和淫羊藿苷远高于茎。

Content Determination of Active Component in Epimedium Koreanum Nakai of Different Pharmaceutical Genetic in Liaoning Province

Objective To determine the content of icariin and flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic in Liaoning Province. Methods Icariin was used as the chemical reference substance to establish HPLC and ultraviolet spectrophotometry methods for content determination of icariin and total flavonoids. Results The content of icariin in leaves was from 0.197% to 0.347%, and in stem was from 0.043% to 0.050%. The content of total flavonids in leaves was from 6.68% to 8.85%, and in stem was from 1.3% to 2.7%. The calibration curves of icariin was in good linearity over the range of 0.3-1.8 μg （r =0.999 9） and total flavordds was 0.5-3 μg/mL （r=0.999 9）. The average recovery of icariin was 99.43% （n =6, RSD=0.29%） and total flavonids was 100.38% （n =9, RSD= 1.60%）. Conclusions The content of flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic has large difference, the content in leaves is far higher than in stem.