虫草多糖对单侧输尿管梗阻小鼠肾小管上皮-间充质转化的影响

目的：观察虫草多糖对单侧输尿管梗阻（UUO）小鼠肾小管上皮一间充质转化（EMT）的影响。方法：建立小鼠UUO模型，虫草多糖干预7d。观察模型及治疗组在第7天时肾间质纤维化程度及肾小管α-平滑肌肌动蛋白（α—SMA）、肾小管上皮表型标记物（E—cadherin）、整合素连接激酶（integrin—linked protein kinase，Iu〈）的表达分布。结果：治疗组的肾间质纤维化程度明显改善，同时α—SMA、ILK蛋白的表达显著低于对照组（P〈0.01）。而E—cadherin的表达显著高于对照组（P〈0．01）。结论：虫草多糖可显著减轻E—cadherin的丢失和α—SMA的表达，并明显抑制ILK的表达，提示其可能通过下调ILK，抑制EMT，从而减轻肾小管间质纤维化。

Effect of Cordyceps Polysaccharide (Cp) on the Transition of Tubular Epithelial Mesenchymal in Mice with Unilateral Ureteral Obstructive

Objective： to investigated the effect of Cp on the transition of tubular epithelial mesenchymal in mice with unilateral ureteral obstruction （UUO）, Methods：Renal interstitial fibrosis was induced by UUO in CD- 1 male mice. The α - SMA,E - eadherin and ILK expressions in renal tissue were observed and analyzed after treatment with Cp for 7 days, Results：The development of interstitial fibrosis was observed to be attenuated in Cp treated renal（ P〈 0.01 ）. With Cp treatment, the expressions of α - SMA and ILK protein were alleviated significantly and loss of E- eadherin expression was inhibited （P〈 0.01 ）. Conclusion： Cp could suppress the loss of E - eadherin and the expression of α- SMA and significantly inhibit the over expression of ILK in obstructed kidney, which indicated it might play an inhibitive role in the progression of EMT through down- regulating of mRNA and protein expressions of ILK.