| 碱性成纤维细胞生长因子对人肝癌细胞蛋白激酶B活性的影响 |
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| 引用本文： | 孙波,颜鑫,李文超,陈剑峰,朱海峰. 碱性成纤维细胞生长因子对人肝癌细胞蛋白激酶B活性的影响[J]. 社区医学杂志, 2012, 10(18): 16-17 |
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| 作者姓名： | 孙波 颜鑫 李文超 陈剑峰 朱海峰 |
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| 作者单位： | 山东省血液中心检验科,山东济南,250014 |
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| 摘 要： | 目的探讨碱性成纤维细胞生长因子(fibroblast growth factor,FGF)对人肝癌细胞(BEL-7402)蛋白激酶B(protein kinase B,PKB)活性的影响。方法以碱性FGF(b FGF)和wortmannin处理人肝癌细胞(BEL-7402),Western blot检测细胞外信号调节蛋白激酶B的活性。结果 25 ng/ml bFGF刺激人肝癌细胞(BEL-7402)10 min,细胞膜和胞质PKB酶活性达高峰。Wortmannin预处理后,人肝癌细胞(Bel-7402)细胞膜和胞质PKB活性在各时间点均明显降低。结论 bFGF能通过磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)途径迅速激活人肝癌细胞(BEL-7402)的PKB活性。
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| 关 键 词： | 肝癌 碱性成纤维细胞生长因子 蛋白激酶B Bel-7402细胞 |
Influence of basic fibroblast growth factor on protein kinase B activity in Bel -7402 cells |
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| SUN Bo , YAN Xin , LI Wen-chao , CHEN Jian-feng , ZHU Hai-feng. Influence of basic fibroblast growth factor on protein kinase B activity in Bel -7402 cells[J]. journal of community medicine, 2012, 10(18): 16-17 |
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| Authors: | SUN Bo YAN Xin LI Wen-chao CHEN Jian-feng ZHU Hai-feng |
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| Affiliation: | SUN Bo, YAN Xin, LI Wen - chao, CHEN Jian - feng, ZHU Hai - feng Department of Laboratory Medicine, Shandong Provincial Blood Center, Jinan Shandong 250014, China |
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| Abstract: | Objective To study the influence of basic fibroblast growth factor（bFGF） on protein kinase B（PKB） activity in Bel - 7402 cells. Methods Bel - 7402 cells were treated with bFGF and wortmannin, and western blot was used to deter- mine their PKB activities afterwards. Results PKB activity in both cell membrane and cytosol reached to the peak at 10 min after being treated by bFGF in a dose of 25 ng/ml. After Wortmannin pretreatment, PKB activities in both cell mem- brane and cytosol were significantly lower at each time point. Conclusions Basic fibroblast growth factor can rapidly acti- vate PKB activity in both cell membrane and cytosol via P13K （phosphatidylinositol 3 -kinase） in Bel- 7402 ceils. |
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| Keywords: | Basic fibroblast growth factor Protein kinase B Wortmannin Bel- 7402 cells |
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