| 苦参素注射液对人食管癌Eca-109细胞周期的影响及机制研究 |
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| 引用本文： | 朱艳琴,王凯娟. 苦参素注射液对人食管癌Eca-109细胞周期的影响及机制研究[J]. 山东医药, 2008, 48(32): 10-12 |
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| 作者姓名： | 朱艳琴 王凯娟 |
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| 作者单位： | 河南中医学院基础医学院,河南,郑州,450008;郑州大学公共卫生学院 |
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| 摘 要： | 目的 探讨苦参素对人食管癌Eca-109细胞增殖周期的影响及其机制.方法 运用流式细胞术检测细胞周期变化和调控细胞增殖周期相关蛋白CyclinD1和CDK4的表达.结果 与阴性对照组和阳性对照组比较,苦参素对Eca-109细胞持续作用48 h后,G0/G1期细胞明显增多,S期细胞明显减少(P均<0.05);CyclinD1和CDK4的表达量均随苦参素剂量增加而减少.结论 苦参素可以明显将人食管癌Eca-109细胞阻滞于G0/G1期,其主要机制可能与CyclinD1及CDK4的低表达有关.
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| 关 键 词： | 食管肿瘤 苦参素 细胞周期 |
Study of the effect and mechanism of cell cycle on the Eca-109 cell with oxymatrine |
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| ZHU Yan-qin,WANG Kai-juan. Study of the effect and mechanism of cell cycle on the Eca-109 cell with oxymatrine[J]. Shandong Medical Journal, 2008, 48(32): 10-12 |
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| Authors: | ZHU Yan-qin WANG Kai-juan |
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| Affiliation: | ZHU Yan-qin1,WANG Kai-juan(1 College of Basic Medical Sciences,Henan College of Traditional Chinese Medicine,Zhengzhou 450008,P.R.China) |
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| Abstract: | Objective To investigate mechanism of oxymatrine affecting Eca-109 cell cycle.Methods The cell cycle and CyclinD1,CDK4 were detected by Flow Cytometry.Results The cells in G0/G1-phase of Eca-109 were higher than those in control groups(P<0.05).The cells in S-phase of Eca-109 were lower than those in control groups(P<0.05).The expression of CyclinD1,CDK4 were reduced depending the dose improvement of oxymatrine(P<0.05).Conclusion Oxymatrine has significantly blocked the cell cycle in G0/G1 phase of the human... |
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| Keywords: | esophageal neoplasms oxymatrine cell cycle |
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