针刺干预缺血性脑卒中大鼠脑内髓鞘碱性蛋白基因的表达

目的观察急性缺血性脑卒中大鼠脑内髓鞘碱性蛋白(MBP)基因的表达情况,探讨针刺疗法干预急性缺血性脑卒中病理过程的机制。方法采用缺血性脑卒中大鼠模型,应用反转录-聚合酶链反应半定量法,观察比较正常组与缺血性脑卒中对照组、早期针刺组、晚期针刺组缺血后1、3、5、7d的MBP基因表达的变化。结果对照组MBP基因的表达随时间先减少后增多,早、晚期针刺组MBP基因的表达不断增强,但早期针刺组增强效果更明显。结论针刺对缺血性脑损伤的保护效应可能与促进脑内MBP基因的表达进而促进髓鞘的再生有关。

Effects of the acupuncture on myelin basic protein gene expression in rats with cerebral arterial thrombosis

OBJECTIVE To observe the effects of myelin basic protein(MBP) gene expression in rats with cerebral arterial thrombosis and the mechanism of the pathology of cerebral arterial thrombosis by the treatment of acupuncture.METHODSThe rats were randomly divided into normal group,control group,acupuncture at early stage group and acupuncture at late stage group.RT-PCR was adopted to observe the change of MBP gene expression in 1,3,5 d and 7 d after cerebral arterial thrombosis.RESULTSAs time went by the MBP expression of control group decreased at first then increased;the MBP expression of both the early and the late acupuncture groups increased,howerer the MBP of early stage group increased intensely.CONCLUSIONAcupuncture has a definite effect in cerebral arterial thrombosis,which may contribute to its action in promoting the expression of MBP,then promoting the regeneration of the myelin sheath.