胃腺癌组织中生存素、核因子和p53基因表达的相关性研究

目的研究凋亡抑制基因生存素、核因子-kB p65和p53基因在胃腺癌组织中的表达情况，并探讨它们与胃腺癌生物学行为之间的关系。方法采用免疫组化S—P法，检测45例胃腺癌组织中生存素、核因子-kB p65及p53基因的表达情况。结果生存素、NF-kB p65和p53基因在胃腺癌组织和正常胃黏膜组织中表达水平差异具有统计学意义（P〈0．01）；生存素的表达情况与胃腺癌浸润深度、淋巴结转移及临床分期密切相关（P〈0．05），而与组织分化程度无关（P〉0．05）；NF-kB p65及p53与胃腺癌的浸润深度、淋巴结转移、临床分期及胃腺癌分化程度密切相关（P〈0．05）。结论胃腺癌组织中生存素、核因子-kB p65及p53基因在胃腺癌的各个阶段均有不同程度的表达，检测三者有助于胃腺癌恶性程度的判定及侵袭转移能力的评估。

The expression of survivin gene nuclear factor-κB p65 and p53 gene in the tissue of gastric adenocarcinoma and their correlation

Objective To investigate the expressions of inhibiting apoptosis gene survivin,NF-KB p65 and p53 gene in the tissure of gastric adenocarcinoma,and their relation to biological behavior. Methods Using immunohistochemical S-P method, the expressions of survivin, NF-KB p65 and p53 gene were examined in 45 patients with gastric adenocarcinoma. Results The expressions of survivin, NF-KB p65 and p53 gene between gastric adenocacinoma and the normal were different. The difference was significant（P 〈 0.01 ）; The expression of survivin was correlated with the degree of the invasion, lymphatic metastasis and clinical stages of gastric adenocarinoma（P 〈 0.05 ） ,but not with differentiation（P 〉 0.05 ） ; The expressions of NF-KB p65 and p53 gene were correlated with the invasion, lymphatic metastasis and clinical stages of gastric adenocarinoma ,and the degree of differentiation, too（P 〈 0.05 ）. Conclusion The survivin, NF- KB p65 and p53 gene in all stage of gastric adenocarinoma have different levels of expression. The detections of survivin, NF-KB p65 and p53 are valuable in evaluating gastric adenocarinoma＇ s malignant degree and its ability of invasion.