| 大鼠肝脏卵圆细胞干细胞特性的研究 |
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| 引用本文： | 李影,赵丽娟,李艳香,施超,陆颖理.大鼠肝脏卵圆细胞干细胞特性的研究[J].上海交通大学学报(医学版),2011,31(5):561-566. |
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| 作者姓名： | 李影 赵丽娟 李艳香 施超 陆颖理 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院内分泌代谢科,上海,200011 |
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| 基金项目： | 上海交通大学医工(理)交叉研究重点基金(YG2009ZD204)~~ |
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| 摘 要： | 目的探讨大鼠肝脏卵圆细胞体外培养扩增及其干细胞特征。方法采用2-乙酰氨基芴/四氯化碳方法建模,胶原酶灌流和流式细胞术分选纯化卵圆细胞,进行培养和扩增。透射电子显微镜观察肝脏卵圆细胞的超微结构,免疫细胞化学法检测干细胞标志物OV-6、c-kit和Thy-1.1的表达;RT-PCR法检测卵圆细胞内c-kit、肝细胞标志物(HNF-4α、AFP)和胆管细胞标志物(CK-7、CK-19)的基因表达;Western blotting法检测OV-6和AFP蛋白的表达。结果大鼠肝脏组织可见明显的卵圆细胞增生,成簇分布,逐渐向肝实质内延伸。细胞体外培养可达40 d以上,细胞体积较小,多呈圆形和卵圆形。透射电子显微镜下可见细胞直径5～10μm,细胞核大,胞质少,核/质比例大,细胞器少,仅见少量线粒体和内质网。免疫细胞化学检测显示,细胞表面OV-6、c-kit和Thy-1.1染色阳性;RT-PCR检测显示,卵圆细胞内有c-kit、HNF-4α、AFP、CK-7和CK-19基因表达;Western blotting检测显示,OV-6蛋白持续表达,AFP蛋白表达逐渐增强。结论大鼠肝脏卵圆细胞体外可大量扩增并且保留其干细胞和双向分化潜...
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| 关 键 词： | 卵圆细胞 增殖 体外 干细胞 |
Characteristics of stem cells from hepatic oval cells in rats |
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| LI Ying,ZHAO Li-juan,LI Yan-xiang,SHI Chao,LU Ying-li.Characteristics of stem cells from hepatic oval cells in rats[J].Journal of Shanghai Jiaotong University:Medical Science,2011,31(5):561-566. |
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| Authors: | LI Ying ZHAO Li-juan LI Yan-xiang SHI Chao LU Ying-li |
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| Institution: | LI Ying,ZHAO Li-juan,LI Yan-xiang,SHI Chao,LU Ying-li(Department of Endocrinology and Metabolism,the Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai200011,China) |
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| Abstract: | Objective To investigate the in vitro culture and characteristics of stem cells of hepatic oval cells in rats.Methods Rats were treated with 2-acetylaminofluorene and carbon tetrachloride to establish the models of hepatic oval cells,two-step collagenase perfusion technique and flow cytometry sorting were used to separate and purify oval cells,and cell culture and amplification were performed.The ultrastructure of hepatic oval cells was observed by transmission electron microscopy,and the expression of stem... |
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| Keywords: | oval cells proliferation in vitro stem cells |
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