育肾养血方治疗卵巢早衰的实验研究

目的探讨全国名老中医朱南孙教授的经验方——育肾养血方治疗卵巢早衰的疗效及作用机制。方法采用75只性周期规律的雌性Wistar大鼠的卵巢组织制备卵巢抗原;另取50只大鼠随机分成正常组(n=10)和模型组(n=40)。模型组采用卵巢抗原皮下注射自身免疫法建立卵巢早衰大鼠模型,造模成功后再分为四组(n=10):中药中、高剂量组(灌服中药复方)、西药对照组(灌服己烯雌酚)、模型对照组(灌服等量生理盐水);正常组为空白对照组(不造模仅灌服等量生理盐水),均连续2周。治疗结束后经腹主动脉采血,留取卵巢组织标本。采用ELISA法检测血清雌二醇(E2)和促卵泡激素(FSH)水平,免疫组织化学法检测卵巢抑制素B(InhB)蛋白阳性表达水平,观察卵巢、子宫湿重变化及组织学改变。结果育肾养血方能提高卵巢早衰大鼠血清E2水平和降低FSH水平;但与模型对照组比较,西药对照组的E2和FSH水平、中药高剂量组的FSH水平差异均有统计学意义(P<0.05)。与模型对照组比较,西药对照组和中药中、高剂量组大鼠卵巢InhB蛋白表达均显著增加(P<0.01);卵巢内淋巴细胞浸润减轻,各级卵泡数增多,卵巢和子宫的湿重增加。结论育肾养血方可以促进卵巢...

Experimental study of treatment of premature ovarian failure with YuShenYangXue decoction

Objective To explore the outcomes and mechanisms of YuShenYangXue decoction designed by Professor ZHU Nan-sun in the treatment of premature ovarian failure. Methods Ovarian antigen was prepared by ovarian tissues of 75 female Wistar rats with regular sexual cycle.Another 50 female Wistar rats were randomly divided into normal group(n=10) and model group(n=40).Rat models of premature ovarian failure were established by autogenous immunization in model group,and were subdivided into 4 groups(n=10): middle-dos...