| p16基因克隆及其对结肠癌细胞SW480生长的抑制作用 |
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| 引用本文： | 孟庆凯,张菁茹,王辉. p16基因克隆及其对结肠癌细胞SW480生长的抑制作用[J]. 上海交通大学学报(医学版), 2011, 31(6): 744-747. DOI: 10.3969/j.issn.1674-8115.2011.06.014 |
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| 作者姓名： | 孟庆凯 张菁茹 王辉 |
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| 作者单位： | 辽宁省肿瘤医院肠外科,沈阳,110042 |
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| 基金项目： | 辽宁省自然科学基金(20092071)~~ |
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| 摘 要： | 目的探讨p16基因对结肠癌细胞生长的抑制作用。方法采用亚克隆技术将p16基因克隆入真核表达载体pEGFP-N3中,Lipofectamine法将p16基因转染入结肠癌细胞株SW480。采用MTT法检测未转染细胞(SW480组)、转染空质粒细胞(SW480-GFP组)和转染p16细胞(SW480-p16组)培养24、48和72 h后细胞的增殖率;Real-Time PCR和Western blotting分别检测3组细胞的p16、CDK4、cyclin D1 mRNA和蛋白的表达情况。结果 p16基因克隆入真核表达载体,并成功转染结肠癌细胞后,在基因和蛋白质水平均有外源性p16基因表达,与SW480组比较,相对表达量均有显著增加(P<0.01)。MTT检测结果显示:培养48 h和72 h后,SW480-p16组的细胞相对增殖率均显著低于SW480组和SW480-GFP组(P<0.01)。Real-Time PCR检测结果显示:SW480-p16组的p16 mRNA相对表达量接近SW480-GFP组的2.38倍,接近SW480细胞的2.59倍,差异具有统计学意义(P<0.01);SW480-p16组的cyclin...
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| 关 键 词： | p16基因 克隆 结肠癌细胞 D型细胞周期蛋白 周期蛋白依赖性蛋白激酶4 |
Cloning of p16 gene and its role in inhibition of colon cancer SW480 cell growth |
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| MENG Qing-kai,ZHANG Jing-ru,WANG Hui. Cloning of p16 gene and its role in inhibition of colon cancer SW480 cell growth[J]. Journal of Shanghai Jiaotong University:Medical Science, 2011, 31(6): 744-747. DOI: 10.3969/j.issn.1674-8115.2011.06.014 |
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| Authors: | MENG Qing-kai ZHANG Jing-ru WANG Hui |
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| Affiliation: | MENG Qing-kai,ZHANG Jing-ru,WANG Hui(Department of Enteric Surgery,Liaoning Cancer Hospital & Institute,Shenyang 110042,China) |
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| Abstract: | Objective To investigate the inhibitory effect of p16 gene on the growth of colon cancer cell line SW480.Methods p16 gene was cloned into eukaryotic express vector pEGFP-N3 using subclone technique,and the recombinant plasmid was transfected into SW480 cells using Lipofectamine method.MTT assay was performed to analyse the proliferation of cells without transfection(SW480 group),cells transfected with blank plasmid(SW480-GFP group) and cells transfected with p16(SW480-p16 group) after culture for 24,48 and ... |
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| Keywords: | p16 gene clone colon cancer cells cyclin D1 cyclin dependent kinase 4 |
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