| 空间记忆维持期小鼠海马酪氨酸激酶B含量的变化 |
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| 引用本文： | 陆志俊,辛怡纯,陈恳,于布为. 空间记忆维持期小鼠海马酪氨酸激酶B含量的变化[J]. 上海交通大学学报(医学版), 2011, 31(6): 759-763. DOI: 10.3969/j.issn.1674-8115.2011.06.017 |
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| 作者姓名： | 陆志俊 辛怡纯 陈恳 于布为 |
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| 作者单位： | 1. 上海交通大学医学院附属瑞金医院麻醉科,上海,200025
2. 上海交通大学医学院附属瑞金医院卢湾分院麻醉科,上海,200025 |
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| 基金项目： | 卢湾区医学基金(LWXK0902)~~ |
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| 摘 要： | 目的观察Morris水迷宫实验中小鼠记忆维持阶段海马酪氨酸激酶B(trkB)蛋白和mRNA的表达情况。方法将28只C57BL/6品系小鼠分为实验组(n=14)和对照组(n=14),实验组参加水迷宫实验,包括4 d训练和1 d探索实验;对照组仅参加1 d探索实验。记录训练期小鼠的潜伏期、游泳距离和平均游泳速度;探索实验中记录小鼠穿越平台次数和平台象限滞留时间百分比。探索实验结束后采用ELISA法测定小鼠海马trkB蛋白含量,采用Real-Time PCR法测定trkB mRNA表达。结果水迷宫训练期,实验组小鼠潜伏期和游泳距离随训练次数增加逐渐缩短(P<0.05);探索实验中实验组小鼠穿越平台次数和平台象限滞留时间百分比明显高于对照组(P<0.05);两组小鼠海马trkB蛋白和mRNA表达差异均无统计学意义(P>0.05)。结论 Morris水迷宫实验显示,小鼠形成对平台空间位置的长期记忆;但在记忆维持阶段,小鼠海马trkB合成没有增加。
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| 关 键 词： | Morris水迷宫 空间记忆 酪氨酸激酶B |
Content of tyrosine kinase B in hippocampus of mouse during persistence of spatial memory |
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| LU Zhi-jun,XIN Yi-chun,CHEN Ken,YU Bu-wei. Content of tyrosine kinase B in hippocampus of mouse during persistence of spatial memory[J]. Journal of Shanghai Jiaotong University:Medical Science, 2011, 31(6): 759-763. DOI: 10.3969/j.issn.1674-8115.2011.06.017 |
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| Authors: | LU Zhi-jun XIN Yi-chun CHEN Ken YU Bu-wei |
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| Affiliation: | LU Zhi-jun1,XIN Yi-chun2,CHEN Ken2,YU Bu-wei1(1.Department of Anesthesiology,Ruijin Hospital,2.Department of Anesthesiology,Ruijin Hospital Luwan Branch,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China) |
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| Abstract: | Objective To investigate the expression of tyrosine kinase B(trkB) protein and mRNA in hippocampus of mouse during persistence of spatial memory in Morris water maze.Methods Twenty-eight C57BL/6 mice were divided into test group(n=14) and control group(n=14).MWM were performed on mice in test group,which included four trail days and one probe day,and mice in control group only experienced one probe day.During trail days,the latencies,swimming distances and swimming velocities were recorded.On probe day,the ... |
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| Keywords: | Morris water maze spatial memory tyrosine kinase B |
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