冷上清中部分有效成分的检测

目的：通过实验真实掌握冷上清中部分有效成分的含量,明确其实际临床使用价值。方法：采集23袋（200ml/袋）ACD-B血液保存液保存的血液,4h内分离制备新鲜冰冻血浆,各袋均留2份标本;1份同新鲜冰冻血浆一起置-35℃保存,于第3天速融后检测总蛋白、白蛋白、纤维蛋白原、凝血因子X含量;1份置4℃保存21d后作为普通血浆检测总蛋白、白蛋白、纤维蛋白原、凝血因子X含量;新鲜冰冻血浆于第3天取出制备冷沉淀,留取冷上清标本1份检测总蛋白、白蛋白、纤维蛋白原、凝血因子X含量。结果：冷上清中的血浆蛋白和凝血因子含量均明显低于普通血浆。结论：冷上清的使用价值有局限性,临床应用应结合病情考虑输注,不能等同于新鲜冰冻血浆和普通冰冻血浆。

Detection for active ingredients of cold supernatant

Objective：To detect the active ingredients of the cold supernatant so as to know about their actual clinical value.Method：23 bags of whole blood（ACD-B,200ml） were collected,separated and prepared for fresh frozen plasma in 4 hours.Each bag took two samples,one saved with the fresh frozen plasma in refrigerator of-35℃,thawed and checked total protein,albumin,fibrinogen,factor X after 3 days;the other saved in refrigerator of 4℃,thawed and checked total protein,albumin,fibrinogen,factor X after 21 days.The fresh frozen plasma was separated and prepared for cryoprecipitate after 3 days,and samples of the plasma cryoprecipitate supernatant were detectd total protein,albumin,fibrinogen and factor X.Result：Plasma proteins and coagulation factors in the cold supernatant were obviously lower than those in ordinary plasma.Conclusion：The value of the cold supernatant would be limited and has limited clinical use.