肠外营养导致大鼠肝细胞炎症因子及受体基因表达的变化及临床意义

目的 长期应用全胃肠外营养(total patenterl nutrition,TPN)相关肝胆功能损害的发病机制仍不清楚.本研究旨在观察不同营养途径下,大鼠肝脏炎症因子及其受体基因表达的差异,以进一步探讨炎症细胞因子及其受体在TPN相关肝损伤中的作用及町能机制.方法 选择12只雄性SD大鼠,随机分为TPN组(6只)和...

Parenteral Nutrition Leads to Alteration of Inflammation Cytokines and Receptors Gene Expression in the Rat

Objective Total parenteral nutrition has been widely used during the past thirties years. It has been found to be an effective and relatively safe method for supplying energy and nutrients to patients with intestinal dysfunction, however, long-term TPN induces a high rate of liver disease in infants. The goal of this experiment was to further study the role of inflammation eytokines and receptors and the potential mechanism of TPN-induced hepatocyte injury by investigating the differential expresision of the inflammatory cytokine genes and receptors. Methods Twelve infant （22 -28 days old ） male SD rats weighing 90 -100 g were randomly divided into two groups （ n = 6 ）. The control group received an oral diet, while the total parenteral nutrition group received continuous TPN infusion through a silastic catheter inserted in the right jugular vein. After 7 days part of the liver was taken for the examination of inflammatory cytokines and receptors gene expression with the oligo gene microarry to analyse the differential expression of the genes in the rats receiving TPN infusion. Results Compared with the control group, the 7-day TPN group was associated with an up-regulation of ILl r2 IL - Iβ ILl rn IL - 18 TGF - α TGF β1 TGF - β2 TGF -β3 TGF - β1il TNF-sf6 IFN - γbp Ccl4 Cyrl, and a down-regulation of Bmp2 XCR1 FGF10 FGFr2 IL - 15 TGF - βr2 Scyall FGFI IL - 10α LTa Bambi Caspasel Cxell0 FGF16 FGFE1 Fragl TNFsf4. Conclusions The rats after TPN show immune suppression in the liver and a slow immune response. The up-regulation of IL - 1β, TGF -α and IL - 18 may be related to TPN-induced liver injury.