不同鼠龄Wistar大鼠肝组织内网状纤维分布的计量形态学研究

目的　研究不同鼠龄肝网状纤维形态计量的差异。方法　以Gomori银网状纤维染色法及图像分析技术 ,观察并测定了 3月龄 (Ⅰ组 )、13月龄 (Ⅱ组 )、2 5月龄 (Ⅲ组 )、33月龄 (Ⅳ组 )Wistar大鼠肝组织之门管区、中央静脉及窦周隙内网状纤维的灰度、面积比及体密度。结果　门管区网状纤维灰度值在 3月龄组、13月龄组分别与33月龄组差异有显著性 (P <0 0 1、P <0 .0 5 ) ,窦周隙网状纤维面积比及体密度在Ⅱ、Ⅲ、Ⅳ组均分别与Ⅰ组间差异有显著性 (P <0 .0 5、P <0 .0 1) ;每组内网状纤维体密度在门管区中央静脉及窦周隙的百分比在Ⅱ、Ⅲ、Ⅳ组分别与Ⅰ组间差异有非常显著性 (P <0 0 0 1) ,每组内不同部位体密度分布比例 ,以Ⅰ组窦周隙网状纤维体密度百分比最大 ,而在Ⅱ、Ⅲ、Ⅳ组内均以门管区百分比最大。结论　从 13月龄开始老化过程中窦周隙网状纤维有减少 ,33月龄组门管区网状纤维嗜银性有减弱

Morphometric Study of Reticular Fiber of Liver of Wistar Rat at Different Ages

Objective To study the difference of reticular fiber of liver of Wistar rat at different ages. Methods Gray scale, area ratio, volume density (Vv) of reticular fiber of portal area, central vein and perisinusoidal space of liver of Wistar rat were observed by using Gomori silver colouring method and image analysis technique. The animals according to the age were divided into 4 groups: 3 months, 13 months, 25 months and 33 months.Results 1.The difference of gray scale of reticular fiber in portal area between group 1,2 and 4 was significant ( P<0.01, P <0.05)respectively. 2. The difference of area ratio of reticular fiber in perisinusoidal space between group 2,4,3 and 1 was significant ( P<0.01, P<0.01, P <0.05)respectively. 3.The difference of Vv of reticular fiber in perisinusoidal space between group 2,3,4 and 1 was significant respectively( P <0.01). 4.In each group the difference of the percentage of Vv of reticular fiber in portal area or central vein or perisinusoidal space between group 2,3,4 and 1 was very significant respectively( P <0.001). 5. In group 1 the percentage of Vv of reticular fiber in perisinusoidal space was the maximal, and central vein the minimal. 6. But in group 2 or 3 or 4 the percentage of Vv in portal area was the maximal, while central vein the minimal.Conclusion Compared with group 3 months, the reticular fiber of perisinusoidal space in groups 13 months, 25 months and 33 months was decreased. The argyrophilia of reticular fiber of portal area in 33 months was reduced.