重组人角质细胞生长因子异构体对兔角膜碱烧伤的疗效观察

目的:观察重组人角质细胞生长因子异构体(简称K102)对实验性兔角膜碱烧伤的治疗作用。方法:60只新西兰大耳白兔,均选左眼为实验眼,随机分成5组,各组均以1mol·L-1的氢氧化钠溶液建立碱烧伤模型。其中,A、B、C组为治疗组(浓度分别为0.5、0.25、0.125mg·mL-1K102滴眼液),D组为阳性对照组(贝复舒滴眼液),E组为阴性对照组(K102滴眼液的溶媒)。镜下观察各组角膜上皮愈合速度(率)及血管新生(CNV)发生情况。结果:在碱烧伤24h内,A、B、C组的角膜上皮生长速率分别为1.52、1.57、1.46mm2·h-1,与E组(0.98mm2·h-1)比较具有显著性差异(P<0.01);各组上皮愈合率各时间点比较治疗组均优于阴性对照组并有显著性差异(P<0.01或<0.05)。治疗组低浓度可抑制CNV,高浓度可促进CNV。结论:K102滴眼液不同浓度均能够显著促进兔角膜碱烧伤后上皮愈合,抑制角膜CNV生长,改善角膜碱烧伤预后。

Clinical Efficacy of Recombined Human Keratinocyte Growth Factor Variant for Alkali Burn in Rabbit

OBJECTIVE： To investigate the effect of recombined human keratinocyte growth factor variant （K102） on corneal alkali burn in rabbit. METHODS： 60 New Zealand rabbits were randomized to 5 groups. Corneal alkali burn models were made and in the left eyes of the rabbits with 1 mol·L^ -1 NaOH solution. Then the trial groups （groups A, B and C） were treated with 0.5, 0.25, 0.125 mg·mL^-1 K102 eye drops, respectively; group D （positive control group） with bFGF eye drops, and group E （negative control group） with solvent of K102 eye drops. The rate of corneal reepithelialization and the area of neovascularization （CNV） were observed under microscope. rate in the trial groups （A, B and C） were 1.52, 1.57 and 1.46 mm2·h^-1,respectively, which were significantly different as compared with negative control group （0.98 mm^2·h^-1） （P〈0.01）, and the epithelium healing rates at different time were all significantly higher than in negative control group （P〈0.01 or P〈0.05） . The low concentration K102 could inhibit CNV, while high concentration conld promote CNV. CONCLUSION： Local application of K102 eye drops at different concentration could enhance the cornea wound healing process in rabbits after corneal alkali burn, inhibit CNV and improve the prognosis of corneal alkali burn.