三个群体MICA基因外显子2、3和4的多态性研究

目的 调查上海地区汉族、云南傣族和新疆维吾尔族3个群体MICA基因外显子2、3和4的多态性。方法 采用聚合酶链反应－序列特异的寡核苷酸探针杂交（polymerase chain reaction and sequence-specific oligonucleotide robing,PCR-SSOP）方法，分析183名汉族、41名傣族和66名维吾尔族正常人群的MICA胞外区等位基因多态性。结果 分别在汉族、傣族和维吾尔族中检测出10、7和9个MICA等位，其中MICA^*008在汉族和维吾尔族中频率最高，而傣族中MICA^*010的频率最高。3个民族MICA等位基因分布方式各不相同，而且维吾尔族的等位基因分布与另外两个民族相比，差异具有显著性。结论 MICA等位基因分布方式具有民族地区特异性。

Analysis on polymorphism in exons 2,3 and 4 of the MICA gene in three different Chinese populations

OBJECTIVE: To detect genetic polymorphism in the exons 2 to 4 of the MICA gene in Chinese Han population in Shanghai, Dai population in Yunnan province and Uygur population in Xinjiang province respectively. METHODS: DNA samples from 183 random healthy individuals in Han population, 41 in Dai population and 66 in Uygur population were genotyped by using the polymerase chain reaction (PCR) and sequence-specific oligonucleotide probing (SSOP) method. RESULTS: In total, 10, 7 and 9 alleles of MICA were observed in Han, Dai and Uygur population respectively. MICA*008 was the most common allele in the population of both Han and Uygur, whereas MICA*010 was the most popular one in Dai population. Han and Dai had a bit similar distribution pattern (Chi-square=12.809 P=0.046), in contrast with Han to Uygur (Chi-square=58.499 P=0) and Dai to Uygur (Chi-square=49.273 P=0). CONCLUSION: MICA gene displayed different allele distributions in different populations.