FL,TPO对人脐带血CD34+细胞体外增殖的影响

目的]研究Flt-3配体（FL）及血小板生成素（TPO）在无血清培养体系中对人脐带血CD34＋细胞体外增殖的影响．方法]采用无血清液体培养法，分别用FL，TPO，FL加TPO作用于体外短期培养脐带血CD34＋细胞，利用其计数及集落分析方法评价增殖效果．结果]分别用FL，TPO，FL加TPO刺激增殖后，人脐带血细胞总数分别增加2．50，5．00，10．10倍，FL加TPO组细胞总数明显高于FL，TPO组；CD34＋细胞分别增殖0．90，1．26，1．61倍；集落总数分别增加2．07，2．27，7．37倍，造血祖细胞CFU-GM产率分别提高3．53，2．40，9．95倍，FL加TPO组产率高于其他组，BFU-E产率分别提高1．45，2．25．6．90倍．结论]在短期无血清液体培养体系中，FL，TPO对人脐带血CD34＋细胞均具有增殖作用，且两者具有协同作用．

Expansion effects of FL, TPO on human umbilical cord blood CD 34 + cells ex vivo

OBJECTIVETo investigate the expansion effects of Flt-3 ligand(FL) and thrombopoietin(TPO) on human umbilical cord blood CD34+ cells.METHODSCD34+ cells from cord blood were cultured with FL and TPO alone or combination with the two cytokines in serum-free liquid culture system.The expansion effects were evaluated with cell count,CD34+ cells and colony assay.RESULTSThe number of nucleated cells increased 2.50,5.00 and 10.10 fold in FL,TPO and FL+TPO group respectively,the cell counts of the combination group were higher than control group significantly.Moreover,the number of nucleated cell in FL+TPO group was significantly higher than that of cells in FL or TPO alone.After expansion,the CD34+ cells increased 0.90,1.26 and 1.61 fold in FL.Clony forming cells(CFC) which remarkable enhanced in all three groups after culture,were increased 2.07,2.27 and 7.37 fold.The number of CFU-GM in FL,TPO and FL+TPO group was significantly higher,increased 3.53,2.40 and 9.95 fold after culture.The yield of BFU-E was increased 1.45 fold and 2.25 fold in FL and TPO group,which enhanced 6.90 fold in FL+TPO group.CONCLUSIONBoth FL and TPO can expand CD34+ cells from cord bloodfairly in short term serum-free liquid culture,the comnation with FL and TPO showed remarkable synergistic effect.