山东省艾滋病流行特征及流行趋势分析

目的 了解山东省艾滋病的流行特征、感染来源及发展规律。为制定山东省艾滋病的防治目标和策略提供依据。方法 采用酶联免疫吸附试验（ＥＬＩＳＡ）、免疫印迹试验（ＷＢ），对高危重点人群血液标本进行人免疫缺陷病毒（ＨＩＶ）抗体检测，对抗－ＨＩＶ阳性标本进行个案流行病学调查，同时重新采集血液标本核实感染者，并从防凝全血中分离单核细胞（ＰＢＭＣ），提取前病毒ＤＮＡ，经多聚酶链反应扩增，进行基因序列测定和亚型分析

Analysis on the characteristic and trend of AIDS epidemics in Shandong

OBJECTIVE: To analyse the data thru labs and field epidemiological investigation from 1987 to 1999, in order to find out the characteristics, sources of infections and its trend which would lead to the development of strategy, prevention and control of AIDS in Shandong. METHODS: Anti-HIV antibody was detected by ELISA and WB in the serum of high-risk groups. When Anti-HIV antibody was positive, personal and epidemiological history was investigated. The anticoagulated blood was repeatedly collected to verify the infected people. PBMCs were separated from the anticoagulated blood to collect previrus DNA. Gene sequence was then detected and subtype analysed by PCR using ABI company kit. RESULTS: Eight hundred five thousand eight hundred and one specimens were detected in the whole province from 1987 to 1999. 52 cases showed Anti-HIV antibody positive, with a rate of 0.06 per thousand. Of the 52 cases, 2 died. Sequence detection and subtype analysis of PBMC in 24 infected people showed that there were five HIV-1 subtypes M: A, B, B', C, E which were from 7 cities of the province. Blood and sexual transmission took the majority with proportions, 59.62% and 30.76% respectively. The subtypes from 13 blood donors were all B'. Among 10 labourers refurned from abroad and 1 spouse six cases were C, two were B, and another two were A and E subtypes respectively. Epidemiologic data showed that most of the infected people were mobile, making sexual transmission easy to occur. CONCLUSION: The moods of HIV/AIDS transmission in Shandong were mainly through blood and sexual contacts.