| Fura－2荧光测定中药有效成分对神经细胞内Ca~（2＋）浓度的变化 |
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| 引用本文： | 韩喻美. Fura－2荧光测定中药有效成分对神经细胞内Ca~（2＋）浓度的变化[J]. 江西医学院学报, 1996, 0(4) |
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| 作者姓名： | 韩喻美 |
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| 作者单位： | Department of Biochemistry，Jiangxi Medical college Nangchang 330006 |
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| 摘 要： | 将中药有效成分黄芩甙、豆腐果甙、金丝桃甙、熊果酸与大白鼠神经细胞一起进行温育，应用新型钙离子荧光指示剂Ｆｕｒａ－２／ＡＭ测定神经细胞内游离Ｃａ２＋浓度变化。结果表明，上述中药有效成分均能影响胞内Ｃａ２＋释放和胞外Ｃａ２＋内流。从而有效地保护神经元免受兴奋性毒性作用
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| 关 键 词： | 中药／药理学 钙通道阻滞剂 光谱测定法.荧光 脑.动物.实验 大鼠 |
Effect of Traditional Chinese Medicine on Ca 2+ Content in Neurons of Rat Tested by Fluorescent Medhod (Fura 2) |
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| Han Yumei. Effect of Traditional Chinese Medicine on Ca 2+ Content in Neurons of Rat Tested by Fluorescent Medhod (Fura 2)[J]. Acta Academiae Medicinae Jiangxi, 1996, 0(4) |
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| Authors: | Han Yumei |
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| Abstract: | In order to study the effect of traditional Chinese medicine on Ca 2+ Content in neurons, effective ingredients of traditional Chinese medicine Baical, helicid Hel.hyperin and Ursolic acid were added into cnltures of rat's neuron and a new fluorescent indicating systen (Fura 2/AM) was used to observe the changes in Ca 2+ content, The results indicated that the above effective ingredients influenced the release of intracellular Ca 2+ and inflow of extracellular Ca 2+ and thus effectively protected the neurons from excitation toxicity. |
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| Keywords: | traditional chinese medicine/pharmacology calcium chamel blockers spectrometry fluorescence brain animals laboratory rats |
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