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| 磁性纳米Fe_3O_4颗粒对藤黄酸诱导的U937细胞凋亡效应的影响(英文) | |
| 引用本文： | 梁艺琼,陈宝安,吴玮玮,高峰,夏国华,邵泽叶,程坚,丁家华,高冲,李国宏,陈文姬,陈宁娜,许文林,孙新臣,刘莉洁,李小毛,王雪梅.磁性纳米Fe_3O_4颗粒对藤黄酸诱导的U937细胞凋亡效应的影响(英文)[J].中国实验血液学杂志,2010,18(1):67-73. |
| 作者姓名： | 梁艺琼 陈宝安 吴玮玮 高峰 夏国华 邵泽叶 程坚 丁家华 高冲 李国宏 陈文姬 陈宁娜 许文林 孙新臣 刘莉洁 李小毛 王雪梅 |
| 作者单位： | 1. 东南大学临床医学院附属中大医院血液科,江苏南京,210009 2. 江苏大学人民医院血液科,江苏镇江,212002 3. 东南大学中大医院肿瘤科,江苏南京,210009 4. 东南大学生理教研室,江苏南京,210009 5. 萨尔大学物理系,德国萨尔布吕肯,D-66041 6. 东南大学生物电子学国家重点实验室,江苏南京,210096 |
| 基金项目： | supported by National 863 Program Emphasis Project(Nanometer Biology Ware Study,No.2007AA0222007); National Nature Science Foundation of P.R.China(No.30740062 and No.30872970); High School DoctorSubject Special-purpose Scientific Research Foundation(No.20070286042) |
| 摘 要： | 本研究探讨联合应用藤黄酸(GA)和磁性纳米Fe3O4颗粒(MNP-Fe3O4)治疗白血病的潜在可能性。采用MTT法检测GA对U937的细胞毒作用及GA联合MNP-Fe3O4对U937细胞生长抑制的影响;流式细胞术检测细胞凋亡率;应用Wright及DAPI染色观察凋亡细胞的形态学变化;采用实时定量RT-PCR及Western blot法分别检测细胞的caspase-3、bcl-2、bax、NF-κB和survivin基因及相应蛋白的表达。结果表明:GA对U937细胞的生长抑制作用呈现时间及剂量依赖性;MNP-Fe3O4本身无细胞毒作用,但能使GA对U937细胞的生长抑制作用增强;联合应用GA和MNP-Fe3O4诱导的细胞凋亡率较单用GA明显增加,细胞呈现典型的凋亡形态改变,同时可见caspase-3及bax表达上调,而凋亡抑制基因如bcl-2、NF-κB和survivin表达下调。结论:MNP-Fe3O4能增强GA对U937细胞的凋亡诱导作用,两者联合应用可能是一种新型而安全有效的白血病治疗方法。 |
| 关 键 词： | 藤黄酸 磁性纳米Fe3O4颗粒 细胞凋亡 U937细胞 |
Effects of Magnetic Nanoparticle of Fe3O4 on Apoptosis Induced by Gambogic Acid in U937 Leukemia Cells |
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| LIANG Yi-Qiong,CHEN Bao-An,WU Wei-Wei,GAO Feng,XIA Guo-Hua,SHAO Ze-Ye,CHENG Man,DING Jia-Hua,GAO Chong,LI Guo-Hong,CHEN Wen-Ji,CHEN Ning-Na,XU Wen-Lin,SUN Xin-Chen,LIU Li-Jie,LI Xiao-Mao,WANG Xue-Mei.Effects of Magnetic Nanoparticle of Fe3O4 on Apoptosis Induced by Gambogic Acid in U937 Leukemia Cells[J].Journal of Experimental Hematology,2010,18(1):67-73. | |
| Authors: | LIANG Yi-Qiong CHEN Bao-An WU Wei-Wei GAO Feng XIA Guo-Hua SHAO Ze-Ye CHENG Man DING Jia-Hua GAO Chong LI Guo-Hong CHEN Wen-Ji CHEN Ning-Na XU Wen-Lin SUN Xin-Chen LIU Li-Jie LI Xiao-Mao WANG Xue-Mei |
| Institution: | (Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical School, Nanjing 210009, Jiangsu Province, China; I Department of Hematology, People Hospital, Jiangsu University, Zhenjiang 212002, Jiangsu Province, China; ZDepartment of Oncology, Zhongda Hospital, Southeast University Clinical Medical College Nanjing 210009, Jiangsu Province, China; 3Department of Physiology, Southeast University, Nanjing 210009, Jiangsu Province, China; 4Department of Physics, University of Saarland , D-66041 Saarbruechen , Germany; 5National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Naniine 210096. Jianesu Province. China) |
| Abstract: | This study was aimed to explore the potential therapy of Gambogic acid(GA) combinated with magnetic nanoparticle of Fe3O4(Fe3O4-MNP) on leukemia.The proliferation of U937 cells and the cytotoxcity were evaluated by MTT assay.Cell apoptosis was observed and analyzed by microscopy and flow cytometry respectively.The expressions of gene and protein were detected by quantitative real-time polymerase chain reaction and Western blot respectively.The results showed that GA enhanced the cytotoxicity for U937 cells ... |
| Keywords: | gambogic acid magnetic nanoparticle Fe3O4 apoptosis U937 cell |
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