成年兔成纤维细胞在纤维蛋白胶表面生长的初步观察

目的:观察成纤维细胞(Fibroblast,FB)复合在纤维蛋白胶表面后,成纤维细胞的生长状态。方法:体外分离、培养、鉴定成年兔皮肤成纤维细胞,并通过倒置显微镜、光学显微镜对成纤维细胞在纤维蛋白胶表面的生长情况进行形态学观察,免疫组化方法检测细胞波形蛋白及角蛋白的表达,鉴定细胞。采用SPSS12.0软件包对数据进行统计学分析。结果:兔成纤维细胞在纤维蛋白胶表面铺展形态佳,增殖速度快。细胞生长2～4h贴壁,3d时细胞呈漩涡状,基本完全融合,约5d后细胞逐渐呈复层生长。免疫组化波形蛋白阳性,角蛋白阴性,差异有显著性(P<0.05),证实细胞来源可靠。结论:兔成纤维细胞在纤维蛋白胶表面生长、增殖良好,纤维蛋白胶有望成为成纤维细胞生长的支架材料。

Preliminary observation of adult rabbit fibroblasts which were seeded on the fibrin glue

PURPOSE:To observe the state of fibroblasts which were seeded on the fibrin glue.METHODS:Adult rabbit skin fibroblasts were isolated,cultured and identified in vitro.Cellular morphologies of fibroblasts which were seeded on the fibrin glue were observed under invert microscope and optical microscope,and the cells were identified by vimentin immunostaining analysis.The data were analyzed using SPSS 12.0software package.RESULTS:Rabbit fibroblasts had good expanding form and proliferated soon on the fibrin glue.The cells grew adherently on the fibrin glue in about 2 to 4 hours,at the 3rd day most of the cells converged into pieces,presenting whirlpool shape.After 5 days,duplicate layers cells gradually formed.The cells were positively stained for vimentin and negative for cytokeratin.The difference was significant(P<0.05) .CONCLUSIONS:Rabbit fibroblasts could grow and proliferate on the fibrin glue well.Fibrin glue may be a suitable material as a scaffold compounded with fibroblasts.