| 艾芬地尔对大鼠局灶性脑缺血损伤后脑水肿和AQP4表达的影响 |
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| 引用本文： | 孟飞,衣服新,叶志军,贾媛媛. 艾芬地尔对大鼠局灶性脑缺血损伤后脑水肿和AQP4表达的影响[J]. 神经解剖学杂志, 2010, 26(2) |
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| 作者姓名： | 孟飞 衣服新 叶志军 贾媛媛 |
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| 作者单位： | 1. 辽宁医学院,附属一院神经外科,锦州,121001
2. 辽宁医学院,生理学教研室,锦州,121001 |
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| 摘 要： | 目的:研究大鼠脑缺血后水通道蛋白4(AQP4)在脑内的表达及N-甲基-D-天冬氨基酸(NMDA)受体拮抗剂艾芬地尔对其的调节作用。方法:线栓法制作大脑中动脉阻塞(middle cerebra lartery occlusion,MACO)局灶性脑缺血模型;干湿重法测量脑组织含水量以评估脑水肿的程度;免疫组化,免疫蛋白印记法测量AQP4(水通道蛋白4)的表达。结果:与假手术组比较,模型组和艾芬地尔组脑组织含水量、梗死灶周围AQP4表达明显增加;与模型组比较,艾芬地尔组能显著减少脑组织含水量,减轻梗死灶周围AQP4表达,差异有统计学意义。结论:缺血损伤后脑组织的AQP4表达上升,脑水肿明显,给予艾芬地尔后可抑制AQP4的表达,减轻脑水肿。
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| 关 键 词： | 艾芬地尔 脑缺血 脑水肿 水通道蛋白4 |
The effect of ifenprodil on cerebral edema and AQP4 expression after the focal brain ischemia reperfusion injury in rat |
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| Meng Fei,Yi Fuixin,Ye Zhijun,Jia Yuanyuan. The effect of ifenprodil on cerebral edema and AQP4 expression after the focal brain ischemia reperfusion injury in rat[J]. Chinese Journal of Neuroanatomy, 2010, 26(2) |
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| Authors: | Meng Fei Yi Fuixin Ye Zhijun Jia Yuanyuan |
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| Affiliation: | Meng Fei1,Yi Fuixin1,Ye Zhijun1,Jia Yuanyuan2(1. Department of Neurosurgery,The First Affiliated Hospital,2. Department of Physiology,Liaoning Medical College,Jinzhou 121001,China) |
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| Abstract: | Objective:To study the effect of ifenprodil,a N-methyl-D-aspara gine (NMDA) receptor antagonist,on brain edema after transient brain focal ischemia in the rats. Methods:The transient focal isehemia was induced by middle cerebral artery occlusion (MCAO); Brain water content (BWC) was measured by dry-wet weighing method; The expression of aquaporin 4 (AQP4) was assessed by immunocytochemistry and Western Blot technique. Results:Compared with sham operation group,BWC and the expression of AQP4 increased obviou... |
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| Keywords: | ifenprodil cerebral ischemia cerebral edema AQP4 |
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