促炎细胞因子对寻常性银屑病患者外周血淋巴细胞表达DARC的影响

目的研究促炎性细胞因子对寻常性银屑病患者外周血淋巴细胞表达达菲抗原趋化因子受体(Duffy antigen/receptor for chem okines,DARC)的影响,探讨DARC在银屑病发病机制中的作用。方法分离、纯化寻常性银屑病患者外周血淋巴细胞,用促炎性细胞因子IL-1β、IL-6或TNF-α刺激,应用FACS技术和RT-PCR方法检测寻常性银屑病患者外周血淋巴细胞DARC蛋白和mRNA表达。结果寻常性银屑病患者外周血淋巴细胞在受到促炎性细胞因子IL-1β、IL-6或TNF-α刺激后,细胞表面DARC蛋白的表达明显降低(P<0.01),细胞内DARC mRNA表达增加。其中以进行期寻常性银屑病患者外周血淋巴细胞DARC表达的改变更为明显。结论促炎性细胞因子能调节银屑病患者外周血淋巴细胞表面DARC蛋白和细胞内DARC mRNA的表达。

The Effects of the Proinflammatory Cytokines on the Expression of DARC on Peripheral Blood Lymphocytes of Patients with Psoriasis Vulgaris

Objective To determine the effects of the proinflammatory cytokines on the expression of DARC on peripheral blood lymphocytes of patients with psoriasis vulgaris. Methods Lymphocytes were isolated and purified from the rest or active psoriasis vulgaris patients, and from healthy controls. DARC protein on peripheral blood lymphocytes was detected by flow cytometry using FITC-labeled DARC mAb. RT-PCR technique was applied to semi-quantitatively analyze DARC mRNA expression in peripheral blood lymphocytes. Results The expression of DARC protein on lymphocytes from psoriasis vulgaris patients were decreased after stimulated with proinflammatory cytokines IL-1β, IL-6 or TNF-α. Enhanced expression level of DARC mRNA could be seen in lymphocytes derived from healthy controls or the psoriasis vulgaris patients. The change of the expression of DARC on lymphocytes was most manifest after stimulated with IL-1β.Conclusion The expression of DARC on lymphocytes derived from the psoriasis vulgaris patients was modulated by different proinflammatory cytokines.