灵芝三萜对小鼠非酒精性脂肪肝的治疗作用

目的研究灵芝三萜（ganoderma triterpene，GT）对小鼠非酒精性脂肪肝的治疗作用。方法以复方高脂饲料连续喂养小鼠5周，建立非酒精性脂肪肝小鼠模型。除正常对照组的8只小鼠外，将造模成功小鼠40只随机分为5组，分别为模型对照组、阳性药物组（壳脂胶囊100mg·kg^-1，1次·d^-1）、GT高、中、低（200，100，50mg·kg^-1，1次·d^-1）剂量组。连续给药6周后，检测血清和肝脏中生化指标，做肝脏组织病理学检查。结果非酒精性脂肪肝小鼠经GT治疗后，GT高、中剂量组都表现出降低非酒精性脂肪肝小鼠血清中总胆固醇（TC）、甘油三脂（TG）含量和天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活性的作用，降低非酒精性脂肪肝小鼠肝脏中TG、TC、丙二醛（MDA）含量，升高超氧化物岐化酶（SOD）活力的作用（P〈0．05或P〈0．01）；组织病理学检查发现脂肪肝程度明显减轻，肝细胞坏死程度减轻。结论GT对高脂饲料所致非酒精性脂肪肝小鼠具有保肝调脂作用。

Therapeutical Effect of Ganoderma Triterpene on Non-alcoholic Fatty Liver in Mice

OBJECTIVE To investigate the therapeutic effect of ganoderma triterpene(GT) on non-alcoholic fatty liver in mice. METHODS A model of the non-alcoholic fatty liver was constructed by feeding mice with high glucose and fatty diet for 5 weeks. Forty mice models with non-alcoholic fatty liver were randomly divided into five groups, model groups, Kezhi capsule groups(100 mg·kg^-1), GT groups (200, 100, 50 mg·kg^-1). The therapeutic effects of GT on non-alcoholic fatty liver and histopathological changes of mice liver were observed. RESULTS Compared with the non-alcoholic fatty liver model group, after treatment with GT, the mice serum levels of total cholesterol(TC), triglyceride(TG), alanine transaminase(ALT) and aspartate transaminase(AST) were significantly decreased(P<0.05 or P<0.01). The hepatic tissue contents of TC, TG and malondialdehyde(MDA) also significantly decreased (P<0.05 or P<0.01). The superoxide dismutase(SOD) activity was higher than that of mice in model group (P<0.05 or P<0.01). And the pathobiology of liver dramatically showed the repairing of hepatic steatosis. CONCLUSION GT has the hypolipideic function with liver protection on high fat diet-induced non-alcoholic fatty liver disease in mice.