| 十二碳烯酸对人H4细胞毒性作用研究 |
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| 引用本文： | 俞发荣,连秀珍,张红梅,李世平,谢明仁. 十二碳烯酸对人H4细胞毒性作用研究[J]. 中国现代应用药学, 2014, 31(2): 134-136 |
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| 作者姓名： | 俞发荣 连秀珍 张红梅 李世平 谢明仁 |
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| 作者单位： | 甘肃政法学院，甘肃省证据科学研究与应用重点实验室，兰州 730070;甘肃政法学院，甘肃省证据科学研究与应用重点实验室，兰州 730070;甘肃政法学院，甘肃省证据科学研究与应用重点实验室，兰州 730070;甘肃政法学院，甘肃省证据科学研究与应用重点实验室，兰州 730070;甘肃政法学院，甘肃省证据科学研究与应用重点实验室，兰州 730070 |
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| 基金项目： | 兰州市科技计划资助项目(2010-1-128) |
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| 摘 要： | 目的探讨十二碳烯酸对人H4细胞的毒性作用机制。方法采用MTT法、流式细胞仪检测十二碳烯酸对人H4细胞的毒性作用。结果分别给予人H4细胞0．3，0．9，2．7mg·L^-1十二碳烯酸培养48h，十二碳烯酸对人H4细胞增殖抑制率分别为41．64％，70．97％，81．52％，半数抑制浓度（IC50）为0．40mg·L^-1，与对照组相比，差异有统计学意义（P〈0．01）；凋亡率分别为10．16％，18．22％，24．06％。结论十二碳烯酸对人H4细胞具有显著的毒性作用，其机制可能与损伤线粒体结构，抑制细胞分裂等有关。
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| 关 键 词： | 十二碳烯酸 人H4细胞 毒性作用 |
| 收稿时间： | 2013-04-16 |
| 修稿时间： | 2013-08-16 |
Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells |
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| YU Farong,LIAN Xiuzhen,ZHANG Hongmei,LI Shiping and XIE Mingren. Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells[J]. The Chinese Journal of Modern Applied Pharmacy, 2014, 31(2): 134-136 |
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| Authors: | YU Farong LIAN Xiuzhen ZHANG Hongmei LI Shiping XIE Mingren |
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| Affiliation: | Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China;Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China;Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China;Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China;Gansu Institute of Political Science and Law, Key Laboratory of Evidence of Science and Technology Research and Application, Lanzhou 730070, China |
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| Abstract: | OBJECTIVE To study 2-dodecenoic acid toxicity mechanism on human H4 cells. METHODS Toxic effects of 2-dodecenoic acid on human H4 cells were detected with methods of MTT and flow cytometry. RESULTS Given the human H4 cells 0.3, 0.9, 2.7 mg·L-1 2-dodecenoic acid for 48 h, human H4 cells proliferation inhibition rates were 41.64%, 70.97%, 81.52%, respectively, and the 50% inhibitory concentration(IC50) was 0.40 mg·L-1, compared with the control group, the difference was statistically significant(P<0.01); apoptosis rates were 10.16%, 18.22%, 24.06%, respectively. CONCLUSION 2-Dodecenoic acid on human H4 cells has significant toxic effects, its mechanism may be related to the destruction of the cell mitochondrial structure and inhibition of cell division. |
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| Keywords: | 2-dodecenoic acid human H4 cells toxic effects |
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