| 益心口服液对化学性缺氧心肌细胞的保护作用 |
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| 引用本文： | 吕嵘,赵培,章忱,卫洪昌.益心口服液对化学性缺氧心肌细胞的保护作用[J].上海中医药大学学报,2009(4):59-61. |
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| 作者姓名： | 吕嵘 赵培 章忱 卫洪昌 |
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| 作者单位： | 上海中医药大学基础医学院; |
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| 基金项目： | 上海市教委重点科研基金资助项目(05zz12);;上海市教委重点学科资助项目(J50301);;上海高校中医内科学E-研究院项目(E03008) |
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| 摘 要： | 目的：探讨益心口服液对化学性缺氧心肌细胞的保护作用。方法：改良Simpson法培养新生大鼠心肌细胞，随机分为生理盐水对照组、缺氧模型组、复方丹参滴丸组及益心口服液大中小3个剂量组。采用二亚硫酸钠造心肌细胞化学性缺氧，并给予相应的药物干预。检测超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）和丙酮酸激酶（PK）含量，观察益心口服液对化学性缺氧心肌细胞的影响。结果：与对照组心肌细胞相比，缺氧模型组心肌细胞变大，折光性差，胞核暗淡，细胞LDH和PK含量降低，细胞MDA含量增加，SOD活性降低（P〈0．05）。复方丹参滴丸组细胞PK含量降低，细胞MDA含量增加，SOD活性降低（P〈0．05）。益心口服液各剂量组SOD活性增加，LDH和PK含量增加，而MDA含量降低（P〈0．05）。未见剂量依从性。结论：益心口服液通过抗氧自由基，稳定细胞膜；降低心肌耗氧量，增加氧供需比等来减少缺氧心肌细胞损伤。
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| 关 键 词： | 益心口服液 心肌细胞 乳酸脱氢酶 丙酮酸激酶 超氧化物歧化酶 丙二醛 |
Protective Effect of "Yixin Oral Liquid" Against Hypoxia Injury in Cultured Rat Cardiac Myocytes |
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| LV Rong ZHAO Pei ZHANG Chen WEI Hong-chang Basic Medical College,Shanghai University of Traditional Chinese Medicine.Protective Effect of "Yixin Oral Liquid" Against Hypoxia Injury in Cultured Rat Cardiac Myocytes[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2009(4):59-61. |
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| Authors: | LV Rong ZHAO Pei ZHANG Chen WEI Hong-chang Basic Medical College Shanghai University of Traditional Chinese Medicine |
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| Institution: | (Basic Medical College, Shanghai University of Traditional Chinese Medicine ) |
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| Abstract: | Objective:To observe the protective effects of "Yixin Oral Liquid"(YOL)against hypoxia injury in cultured rat cardiac myocytes. Methods: The myocardial cells were cultured by improved Simpson method and randomized into saline control group,hypoxia model group,compound salvia pellet(CSP) group and three YOL groups with different dosages. Then after 24 h effects of serums containing correspondent drugs,the hypoxic damages of cultured myocardial cells was induced by Na2S2O4 except the normal group. After 3 h o... |
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| Keywords: | ＂Yixin Oral Liquid＂ cardiac myocyte lactate dehydrogenase （ LDH ） pymvate kinase （ PK ） superoxide dismutase（SOD） malondialdehyde （MDA） |
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