反义IGF-Ⅰ寡核苷酸转染对人肝癌细胞增生及凋亡的影响

为研究胰岛素样生长因子 I(IGF I)反义寡核苷酸转染对人肝癌细胞增生、分化及凋亡的影响 ,探讨寡核苷酸转染治疗肝癌的可行性 ,利用反义核酸技术 ,合成针对IGF I的寡核苷酸片段 ,利用脂质体包裹反义IGF I寡核苷酸片段瞬时转染人肝癌细胞系BEL 74 0 2细胞 ,MTT法检测细胞增生 ;放免法检测培养细胞上清中AFP、CEA的分泌量 ;采用末端标记 (Tunel)法检测细胞凋亡的变化。结果发现转染反义IGF I寡核苷酸可使人肝癌细胞系BEL 74 0 2细胞增生下降 ,AFP、CEA表达降低 ,凋亡细胞数量增多。反义IGF I寡核苷酸转染人肝癌细胞系BEL 74 0 2细胞可以降低细胞增生、减少细胞去分化并诱导肝癌细胞凋亡

Transfection of Antisense Oligonucleotide Against the Insulin Like Growth Factor I on Proliferation and Apoptosis of Liver Cancer Cell

The study was designed to observe the inhibited effect of the antisense oligonuc leotide(ASON) targeting IGF-I on proliferation,differatiation and apoptosis of the liver cancer cell line BEL-7402 by antisense oligonucleotid e transfection. Antisense oligonucleotide against the insulin like growth factor I was synthesized and transfected to human liver cancer cell line B EL-7402 cells by Lipofectin kits. MTT method was used to measure the cell proli feration. The expression level of CEA and AFP in the supernatant of transfect ce ll culture was measured by RIA. The apoptosis of BEL-7402 cells was observed by TUNEL techniques. It was shown that the lipofectin ASON significantly inhibite d the growth of BEL-7402 cells. The expression of AFP and CEA in the supernatan t of transfected cell culture were significantly lower as compared to the parent cells ( P <0.05 and P <0.01 respectively). Antisense IGF-I oligonucle otide transfected to human liver cancer cell line BEL-7402 can lead to a reduct ion of proliferation and differatiation. It can also induce the apoptosis of cells .