| 蔓荆子药材及其混伪品DNA条形码鉴定研究 |
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| 引用本文： | 张晓存,徐迪,孙伟,宋明,刘霞.蔓荆子药材及其混伪品DNA条形码鉴定研究[J].世界科学技术-中医药现代化,2014,16(11):2366-2370. |
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| 作者姓名： | 张晓存 徐迪 孙伟 宋明 刘霞 |
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| 作者单位： | 武汉理工大学化学化工与生命科学学院 武汉 430070;武汉理工大学化学化工与生命科学学院 武汉 430070;中国中医科学院中药研究所 北京 100700;武汉理工大学化学化工与生命科学学院 武汉 430070;武汉理工大学化学化工与生命科学学院 武汉 430070；中国中医科学院中药研究所 北京 100700 |
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| 基金项目： | 科学技术部国家重大科技专项（2014ZX09304-307-001-020）：中药新药安全性检测技术与标准研究——中药原料药混伪品分子基因鉴定技术与标准，主课题负责人：肖红斌，子课题负责人：刘霞；武汉理工大学自主创新研究基金本科生项目（146720018）：麻黄及麻黄根中麻黄碱及伪麻黄碱的提取工艺研究，负责人：徐迪。 |
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| 摘 要： | 本研究应用ITS2序列鉴别蔓荆子及其混伪品，收集药材和基原植物样本共46份，通过提取基因组DNA、PCR扩增和双向测序获得ITS2序列，经CodonCode Aligner V4.2拼接注释获得序列，应用MEGA5.0对序列进行分析比对，计算种内和种间遗传距离（Kimura 2-Parameter，K2P），构建系统发育NJ树进行鉴定。结果表明，蔓荆子药材基于ITS2序列的种内最大K2P遗传距离均小于其与混伪品的种间最小K2P遗传距离，NJ树显示蔓荆子药材与其混伪品可明显分开，表现出良好的单系性。因此，应用ITS2序列能够准确地鉴定蔓荆子药材及其混伪品。
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| 关 键 词： | 蔓荆子 混伪品 DNA条形码 ITS2 |
| 收稿时间： | 2014/11/5 0:00:00 |
| 修稿时间： | 2014/11/18 0:00:00 |
Identification of Viticis Fructus and Its Adulterants by ITS2 Sequence |
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| Zhang Xiaocun,Xu Di,Sun Wei,Song Ming and Liu Xia.Identification of Viticis Fructus and Its Adulterants by ITS2 Sequence[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2014,16(11):2366-2370. |
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| Authors: | Zhang Xiaocun Xu Di Sun Wei Song Ming and Liu Xia |
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| Institution: | School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China;School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China;School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | ITS2 sequence was used as a barcode to identify Viticis Fructus and its adulterants. The study collected 46 samples include Viticis Fructus and its adulterants. The sequences were obtained by extracting DNA, amplification, sequencing bi-direstionlly, and then assembling with CodonCode Aligner. To identify Viticis Fructus and its adulterants, the study used the methods of computing the genetic distances by Kimura 2-Parameter (K2P) model and constructing the Neighbor-joining (NJ) phylogenetic trees using MEGA5.0. The results showed that the maximum inter-specific K2P distance of Viticis Fructus was less than the minimum inter-specific K2P distance of Viticis Fructus and its adulterants and the NJ tree indicated that the Viticis Fructus and its adulterants could be distinguished clearly. Therefore, using ITS2 barcode can distinguished Viticis Fructus and its adulterants accurately. |
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| Keywords: | Viticis Fructus adulterants DNA barcoding ITS2 |
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