| 高效液相色谱法测定鱼腥草中金丝桃苷与槲皮苷的含量 |
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| 引用本文： | 郑一敏,胥秀英,傅善权,杨艳红.高效液相色谱法测定鱼腥草中金丝桃苷与槲皮苷的含量[J].现代中药研究与实践,2005,19(3):27-28. |
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| 作者姓名： | 郑一敏 胥秀英 傅善权 杨艳红 |
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| 作者单位： | 重庆工学院生物工程学院,重庆,400050;重庆工学院生物工程学院,重庆,400050;重庆工学院生物工程学院,重庆,400050;重庆工学院生物工程学院,重庆,400050 |
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| 摘 要： | 目的用高效液相色谱法测定鱼腥草中金丝桃苷与槲皮苷的含量。方法采用ShimadzuC18柱(150mm×4.6mm,5μm),流动相为甲醇-0.2%H3PO4水溶液(45:55),流速1.0mL.min-1,检测波长350nm,柱温室温,用外标法定量,测定鱼腥草中金丝桃苷与槲皮苷的含量。结果金丝桃苷的线性范围0.50-2.50μg,r=0.9998,回收率102.47%,RSD1.04%;槲皮苷的线性范围0.40-2.00μg,r=0.9996,回收率97.92%,RSD2.13%。结论该方法简便,快速,线性关系良好。
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| 关 键 词： | 鱼腥草 金丝桃苷 槲皮苷 高效液相色谱法 |
| 文章编号： | 1004-2199(2005)03-0027-02 |
| 修稿时间： | 2005年1月21日 |
Quantitative Determination of Hyperoside and Quercitrin in Houttuynia cordata by HPLC |
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| ZHENG Yi-min,XU Xiu-ying,FU Shan-quan,YANG Yan-hong.Quantitative Determination of Hyperoside and Quercitrin in Houttuynia cordata by HPLC[J].Research and Practice on Chinese Medicines,2005,19(3):27-28. |
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| Authors: | ZHENG Yi-min XU Xiu-ying FU Shan-quan YANG Yan-hong |
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| Abstract: | Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata. |
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| Keywords: | Houttuynia cordata Hyperoside Quercitrin HPLC |
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