| 大鼠海马神经细胞原代培养技术的优化 |
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| 引用本文： | 张金波,王淑秋,朱金玲,罗佳滨,张淑红,金岳雷.大鼠海马神经细胞原代培养技术的优化[J].黑龙江医药科学,2009,32(4):20-21. |
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| 作者姓名： | 张金波 王淑秋 朱金玲 罗佳滨 张淑红 金岳雷 |
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| 作者单位： | 佳木斯大学基础医学院,黑龙江,佳木斯,154007 |
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| 基金项目： | 黑龙江省教育厅科技项目资助 |
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| 摘 要： | 目的：建立海马神经细胞原代培养技术。方法：取新生1～3d的Wistar大鼠，开颅，迅速分离出海马组织在Hanks中剪碎，加入0．125％胰蛋白酶2mL消化，胎牛血清终止消化，细胞计数后种植于多聚赖氨酸包被的6孔培养板中。结果：神经细胞存活率高，纯度达90％以上，神经细胞生长良好。结论：用此方法能成功获取大鼠海马神经细胞。
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| 关 键 词： | 海马神经细胞 原代培养 胰蛋白酶 |
Optimization of primary culture technique for rat hippocampal neuron |
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| ZHANG Jin-bo,WANG Shu-qiu,ZHU Jin-ling,LUO Jia-bin,MA Xiao-ru,JIN Yue-lei.Optimization of primary culture technique for rat hippocampal neuron[J].Heilongjiang Medicine and Pharmacy,2009,32(4):20-21. |
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| Authors: | ZHANG Jin-bo WANG Shu-qiu ZHU Jin-ling LUO Jia-bin MA Xiao-ru JIN Yue-lei |
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| Institution: | College of Basic Medicine of Jiamusi University;Jiamusi 154007;China |
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| Abstract: | Objective: To establish primary culture technique for rat hippocampal neuron.Methods:The neonatal Wistar rat(1~3d) were killed,the hippocampal tissue was separated and quickly broken into pieces in Hanks liquid,and then digested in 0.125% trypsinase and ended to be digested with fetal cattle serum.The cells were counted and then grew in culture plate which was coated with polylysine.Results:The neuron livability was high and the pure degree reach 90%,and the growth of neuron were good.Conclusion:Rat hippoca... |
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| Keywords: | hippocampal neuron primary culture trypsinase |
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