芝麻素对四氯化碳慢性肝损伤大鼠肝脏的保护作用

目的观察芝麻提取物芝麻素对四氯化碳(CC l4)慢性肝损伤大鼠肝脏的保护作用。方法将60只SD大鼠随机分为正常组、模型组、芝麻素高剂量组、芝麻素中剂量组、芝麻素低剂量组和甘利欣对照组6组,各10只,除正常组外于皮下注射40%CC l4溶液(5 mL/kg),以后每3 d予40%CC l4溶液(3 mL/kg)皮下注射造模,正常组予等容积花生油皮下注射。同时,正常组、模型组每日予0.9%氯化钠注射液1.5 mL灌胃;芝麻素高剂量组、芝麻素中剂量组、芝麻素低剂量组每日分别予9.0、3.0、1.0 mg/mL浓度芝麻素甲基纤维素悬浊液1.5 mL灌胃;甘利欣对照组每日予0.3 mg/mL甘利欣1.5 mL灌胃。8周后处死大鼠,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总蛋白(TP)、白蛋白(A lb)、球蛋白(G)含量,并进行病理组织学检查。结果芝麻素高剂量组、芝麻素中剂量组大鼠血清ALT、AST活性虽然仍高于正常组,但比模型组明显降低(P0.05),且与甘利欣对照组比较差异无统计学意义(P0.05),而且芝麻素高剂量组、芝麻素中剂量组大鼠肝组织结构破坏不明显,肝细胞脂肪变性程度显著减轻,肝细胞坏死减轻,尤以芝麻素高剂量组肝组织改善显著,接近甘利欣对照组;芝麻素高剂量组、芝麻素中剂量组、芝麻素低剂量组、甘利欣对照组TP、A lb含量比较差异无统计学意义(P0.05),但均较模型组高(P0.05),与正常组比较差异无统计学意义(P0.05),6组血清G含量比较差异无统计学意义(P0.05)。结论芝麻素能改善造模大鼠肝细胞损害和坏死状况,对肝功能有保护作用,而且与剂量有一定关系。

Protective effect of sesamin on carbon tetrachloride-induced chronic liver injury in rats

Objective To observe the protective effect of Sesamin on carbon tetrachloride-induced chronic liver injury in rats.Methods 60 SD rats were randomly divided into six groups,normal group,model group,high-,middle,low-dose sesamin group and Diammonium Glycyrrihizinate group.Beside rats in normal group were given by hypodermic of isasteric arachis oilrats,the chronic hepatic injury model of rats in other groups were induced by hypodermic 40% carbon tetrachloride（CCl4）,meanwhile,9.0 mg/mL,3.0 mg/mL and 1.0 mg/mL dose sesamin methylcellulose suspension was given by intragastric administration in three sesamin groups,and 0.3 mg/mL Diammonium Glycyrrihizinate was given by intragastric administration in control group.The level of glutamic pyruvic transaminase（ALT）,glutamic oxaloacetic transaminase（AST）,total protein（TP）,albumin（Alb）,globulin（G） in serum were observed,the change of hepatic pathohistology in each group was detected.Results Serum ALT,AST in high,middle dose sesamin groups were lower than those in model group（P〈0.05）,but still higher than those in normal group（P〈0.05）.There was no significant difference compared with those in Diammonium Glycyrrihizinate group（P〈0.05）.Serum TP,Alb in high,middle,low dose sesamin groups,Diammonium Glycyrrihizinate group were higher than those in model group（P〈0.05）,but there is no statistically significant differences between high,middle,low dose sesamin groups,Diammonium Glycyrrihizinate group in serum TP and Alb（P〉0.05）.The pathologic damage of hepatic tissue of three sesamin groups was improved in different degrees,and similar to Diammonium Glycyrrihizinate group.Conclusion Sesamin can improve hepatocellular damage of rats with chronic liver injury induced by CCl4,and has obvious protective effect on liver.