| RP-HPLC法测定不同采收期黄花乌头中关附甲素的含量 |
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| 引用本文： | 冯锋,黄剑,刘静涵.RP-HPLC法测定不同采收期黄花乌头中关附甲素的含量[J].中草药,1999,30(7):495-496. |
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| 作者姓名： | 冯锋 黄剑 刘静涵 |
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| 作者单位： | 中国药科大学天然药物化学教研室,南京210009 |
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| 摘 要： | 采用ＲＰ－ＨＰＬＣ方法对黄花乌头Ａｃｏｎｉｔｕｍｃｏｒｅａｎｕｍ抗心律失常有效成分关附甲素的含量进行了测定。实验采用Ｃ１８柱，以乙腈－磷酸三乙胺缓冲液为流上，检测波长２０８ｎｍ，以外标法定量，为合理有效地利用药物资源提供了参考。
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| 关 键 词： | 黄花乌头 关附甲素 RP－HPLC 含量测定 |
| 收稿时间： | 1998/9/21 0:00:00 |
| 修稿时间： | 1998-09-21 |
Assay of Guanfu Base A in Korean Monkshood (Aconitum coreanum) at Different Stages of Reaping by RP-HPLC |
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| Feng Feng,Huang Jian and Liu Jinghan.Assay of Guanfu Base A in Korean Monkshood (Aconitum coreanum) at Different Stages of Reaping by RP-HPLC[J].Chinese Traditional and Herbal Drugs,1999,30(7):495-496. |
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| Authors: | Feng Feng Huang Jian and Liu Jinghan |
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| Institution: | Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009;Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009;Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009 |
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| Abstract: | A method for the determination of guanfu base A, the active anti-arrhythmic principle in Aconitum coreanum (Levl ) Raipaics by RP-HPLC was reported. Results showed that its content was higher in the root collected in August. A good seperation and quantitative determination method was accomplished on an ODS column with UV detector at 208 nm CH,CH_3CN/H_2O-H_3PO_4-NEt_3(2: 8 v/v, PH 3. 04) was used as the mobile phase. |
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| Keywords: | Acontium coreanum (Levl ) Raipaics guanfu base A RP-HPLC |
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