人骨髓间质干细胞和嗅鞘细胞移植对大鼠急性脊髓损伤功能修复的比较研究

目的对比人骨髓基质细胞(BMSCs)与人胚嗅鞘细胞(OECs)移植对大鼠脊髓损伤功能修复的影响。方法将45只SD大鼠分成脊髓损伤后BMSCs移植组(BMSCs组)、OECs移植组(OECs组)和PBS对照组(PBS组),通过BBB评分、运动诱发电位(MEP)评估脊髓传导功能的改善状况,免疫组织化学方法检测移植细胞存活和分化情况,病理形态学方法观察组织结构修复情况。结果BMSCs组BBB评分高于OECs组(P<0.05);两细胞治疗组MEP潜伏期明显缩短(P<0.05);BMSCs组嗜银染色可见脊髓损伤近端有较多再生纤维向远端延伸,形成神经纤维束,而OECs组再生纤维较少;两种移植细胞均可在损伤处部分存活,BMSCs组可见BMSCs来源的细胞Nestin、NF、GFAP的阳性表达。结论BMSCs移植比OECs移植能更有效促进大鼠急性脊髓损伤修复。

Comparison of bone marrow-derived mesenchymal stem cells and olfactory ensheathing cells for the repair of acute SCI in rats

Objective To compare the effect of human bone marrow-derived mesenchymal stem cells (BMSCs) with human olfactory ensheathing cells(OECs) on spinal cord injury (SCI) in rats. Methods Firty-five SD rats were divided into 3 groups: BMSCs group (grafting BMSCs after SCI), OECs group (grafting OECs after SCI) and PBS group (grafting PBS after SCI). The motor function of hind limb and the neural conduction of injured spinal cord were evaluated by Basso-Beattie-Bresnahan (BBB) scale and the latency of motor evoked potentials (MEP). Histological and immunohistochemical methods were used to analyze the change of pathology and verify the survival, migration and differentiation of implants. Results BMSCs group had higher BBB scores than OECs group(P<0.05). MEP latency in BMSCs group and OECs group significantly shortened compared with that in PBS group (P<0.05). Axonal staining showed that there were more regenerated axons stretched toward to distal in the BMSCs group than those in the OECs group. The grafted BMSCs and transplanted OECs could survive in SCI rats. Immunohistochemical staining showed that the transplanted BMSCs partially expressed Nestin, neurofilament (NF) and glial fibrillary acidic protein (GFAP). Conclusion Transplantation with BMSCs seems to be more beneficial to the injured spinal cord repair than with OECs after acute SCI in rats.