新型快速成形的三维多孔支架材料构建组织工程软骨

目的:探讨以骨髓基质细胞(BMSC)为种子细胞,以快速成形的生物相容性材料为支架构建组织工程软骨的可行性.方法:成年兔BMSC在成软骨培养液中诱导培养,使之具有软骨细胞表型后,接种于快速成形的生物相容性三维支架材料聚乳酸/聚羟乙酸共聚物(poly-lactic-co-glycolic acid,PLGA),经体外培养扩增2 wk后植入自体肌袋,术后8 wk取材,进行组织学、甲苯胺蓝染色及II型胶原免疫组织化学观察.结果:成年兔BMSC在体外可诱导为软骨细胞,接种于三维支架材料培养后,扫描电境观察见细胞在支架上可大量扩增;接种细胞的支架植入自体肌袋8 wk,可形成软骨样组织,甲苯胺蓝染色及II型胶原免疫组化染色阳性.结论:具有软骨细胞表型的BMSC接种于快速成形的三维支架材料在体内非关节环境可形成软骨样组织.

Construction of tissue engineered cartilage with bone marrow stromal cells seeding on rapid prototyping poly scaffold

AIM: To investigate the possibility of cartilage engineering using bone marrow stromal cells and rapid prototyping porous poly scaffolds. METHODS: Adult rabbit bone marrow stromal cells (BMSC) were cultured in chondrogenic medium to induce them to express chondrocyte phenotype. The induced cells were implanted onto the rapid prototyping porous poly scaffolds (poly-lactic-co-glycolic acid, PLGA) and cultured for 2 weeks. The cell-loaded scaffolds were implanted into a muscle pocket. The specimens were harvested at 8 weeks after implantation and examined histologically. The tissues were stained with toluidine blue for proteoglycan and immunohistochemically detected for type II collagen. RESULTS: BMSC of adult rabbits cultured in chondrogenic medium expressed chondrocyte phenotype. Scanning electron microscopy showed that BMSC expanded on rapid prototyping porous poly scaffolds. Type II collagen was identified in the cartilaginous tissues. CONCLUSION: The construction of a tissue-engineered cartilage is possible with chondrogenesis induced BMSC seeding on rapid prototyping porous poly scaffolds in a non-joint environment.