| 人血清清蛋白cDNA克隆的免疫酶法筛选及其鉴定 |
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| 引用本文： | 张澄波,马大龙.人血清清蛋白cDNA克隆的免疫酶法筛选及其鉴定[J].首都医学院学报,1989,10(1):15-18. |
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| 作者姓名： | 张澄波 马大龙 |
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| 作者单位： | 首都医学院细胞生物研究室，北京医科大学免疫学教研室，首都医学院细胞生物研究室 |
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| 摘 要： | 报道了用免疫酶法从人肝细胞cDNA文库中筛选清蛋白cDNA克隆的过程。所得到的克隆用免疫学方法及内切酶水解法进行鉴定。在7个阳性克隆中,有8个cDNA克隆分子的叠加占据了整个清蛋白cDNA编码区的长度
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| 关 键 词： | 人血清清蛋白 cDNA克隆 免疫酶法 大肠杆菌 |
| 收稿时间： | 1988-10-06 |
The Screening of cDNA Clones of Human Serum Albumin Using Enzyme Labeled Immuno Assay and Their Identification |
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| Zhang Chengbo.The Screening of cDNA Clones of Human Serum Albumin Using Enzyme Labeled Immuno Assay and Their Identification[J].Journal of Capital University of Medical Sciences,1989,10(1):15-18. |
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| Authors: | Zhang Chengbo |
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| Institution: | Zhang Chengbo Department of Cell Biology,Capital Institute of Medicine |
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| Abstract: | Using enzyme labeled immuno assay, the procedures of screening the clones of albumin cDNA from a library of human hepatic cells were reported. These selected clones were identified with immunological method and res- triction enzymes digestion, of the seven positive clones, the sum of three overlaps the whole length of coding region of albumin. |
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| Keywords: | human serum albumin cDNA clone enzyme labeled immuno assay E Coli |
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